Effects of lead on the male mouse as investigated by in vitro fertilization and blastocyst culture

Journal Article · Sun Feb 01 00:00:00 EST 1987 · Environ. Res.; (United States)

DOI:https://doi.org/10.1016/S0013-9351(87)80015-4· OSTI ID:6486866

Johansson, L; Sjoeblom, P; Wide, M

Long-term exposure of male mice to inorganic lead (lead chloride, 1 g/liter) in the drinking water reduces their fertility. The cause of this reduction, expressed as a decrease in the number of mated females showing inplantations, was investigated, using an in vivo fertilization method. It was found that spermatozoa from lead-exposed males had a significantly lower ability to fertilize mouse eggs than those from unexposed males. Preimplantation embryos, isolated from uterine horns of mice mated with lead-exposed males. Preimplantation embryos, isolated from uterine horns of mice mated with lead-exposed males, were examined. No morphologically abnormal embryos were found. However, when cultured in vitro over the implantation period, blastocysts of the group mated with lead-exposed males showed an increased frequency of delayed hatching from the zona pellucida or an inability to hatch. Among blastocysts from this group a decreased frequency of inner cell mass development was also found.

• Research Organization: Uppsala Univ., Sweden
• OSTI ID: 6486866
• Journal Information: Environ. Res.; (United States), Vol. 42:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
LEAD
TOXICITY
MICE
ONTOGENESIS
REPRODUCTIVE DISORDERS
CHRONIC EXPOSURE
EMBRYOS
FERTILIZATION
IN VITRO
LEAD CHLORIDES
MORPHOLOGICAL CHANGES
ANIMALS
CHLORIDES
CHLORINE COMPOUNDS
ELEMENTS
HALIDES
HALOGEN COMPOUNDS
LEAD COMPOUNDS
LEAD HALIDES
MAMMALS
METALS
RODENTS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology