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Characterization of a cDNA clone coding for a mouse 85 kDa heat shock protein from a 3-methylcholanthrene-induced tumor

Conference · · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
OSTI ID:6485887
; ; ;
Heat shock proteins (hsp) of approx. 85 kDa have been found associated with steroid hormone receptors and with the src oncogene product. Recently, the authors have shown that a mouse tumor-associated transplantation antigen from a 3-methylcholanthrene-induced tumor shares amino acid sequence homology with the 85 kDa hsp. Amino acid sequence of peptides from this antigen were used to synthesize oligonucleotide probes to screen a mouse cDNA library. A cDNA clone coding for a 85 kDa mouse hsp has been isolated and its sequence determined. Predicted amino acid sequences from this cDNA clone share significant homology to the published 90 kDa hsp of Saccaromyces cerevisiae and to the 83 kDa hsp of Drosophila melanogaster. In addition, the predicted amino acid sequence at the carboxyl terminus shares identity with that of the 70 kDa hsp from various species as well as that of the Escherichia coli dnaK gene product. Northern blot analysis indicates that the mouse 85 kDa hsp is coded for by a kb mRNA. The size of the mRNA is indistinguishable between normal and malignant cells.
Research Organization:
National Cancer Institute, Bethesda, MD
OSTI ID:
6485887
Report Number(s):
CONF-8606151-
Conference Information:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
Country of Publication:
United States
Language:
English

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Related Subjects

3-METHYLCHOLANTHRENE
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACID SEQUENCE
ANIMALS
AROMATICS
CARCINOGENESIS
DISEASES
DNA
HYDROCARBONS
MAMMALS
MESSENGER-RNA
MICE
MOLECULAR STRUCTURE
NEOPLASMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PATHOGENESIS
PEPTIDES
POLYCYCLIC AROMATIC HYDROCARBONS
PROTEINS
RNA
RODENTS
VERTEBRATES