A YAC contig of approximately 3 Mb from human chromosome 5q31 [yields] q33

Journal Article · Mon Jan 31 23:00:00 EST 1994 · Genomics; (United States)

DOI:https://doi.org/10.1006/geno.1994.1096· OSTI ID:6483203

Li, Xiang; Wang Jabs, E; Hawkins, A L; Griffin, C A ^[1]; Wise, C A; Lovett, M ^[2]; Le Paslier, D ^[3]; Pittler, S J ^[4]

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1. John Hopkins School of Medicine, Baltimore, MD (United States)
2. Univ. of Texas Southwestern Medical Center, Dallas, TX (United States)
3. CEPH, Paris (France)
4. Univ. of South Alabama College of Medicine, Mobile, AL (United States)

The human chromosome 5q31-q33 region contains an interesting cluster of growth factor and receptor genes. In addition, several genetic disease loci have been localized within this region, but have not as yet been isolated as molecular clones. These include those loci involved in autosomal dominant limb-girdle muscular dystrophy, diastrophic dysplasia, Treacher Collins syndrome, and myeloid disorders associated with the 5q-syndrome. A yeast artificial chromosome (YAC) contig of this region would assist in the further localization and isolation of these genes. The authors have used YACs isolated from the Washington University and Centre d'Etude du Polymorphisme Humain YAC libraries, including YACs from the large insert (mega) YAC library to build a contig greater than 3 Mb in size. An STS content strategy coupled with limited walking from YAC ends was used to isolate 22 overlapping YACs with as much as sixfold coverage. A total of 20 STSs, derived from genes, anonymous sequences, and vector Alu-PCR or inverse PCR products, were used to compile this contig. The order of loci, centromere-GRL-D5S207-D5S70-D5S545-D5S546-D5S547-D5S68-D5S548-D5S210-D5S549-D5S686- ADRB2-D5S559-CSF1R-D5S551-RPS14-D5S519-SPARC-telomere, was derived from the overlapping clones. This contig and clones derived from it will be useful substrates in selecting candidate cDNAs for the disease loci in this interval. 45 refs., 1 fig., 2 tabs.

• OSTI ID: 6483203
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 19:3; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
BIOTECHNOLOGY
BONE MARROW CELLS
CHROMOSOMES
CLONING
CONNECTIVE TISSUE CELLS
DISEASES
DNA HYBRIDIZATION
DNA-CLONING
GENE MUTATIONS
GENETIC ENGINEERING
GENETIC MAPPING
HEREDITARY DISEASES
HUMAN CHROMOSOME 5
HUMAN CHROMOSOMES
HYBRIDIZATION
MAPPING
MUSCLES
MUTATIONS
NUCLEIC ACID HYBRIDIZATION
SOMATIC CELLS