Detection of metal ions by liquid chromatographic separation of their 1,3-dimethyl-4-acetyl-2-pyrazolin-5-one chelates
Metal ions are determined by adding 1,3-dimethyl-4-acetyl-2-pyrazolin-5-one (DMAP) to an aqueous sample and then separating the metal chelates by direct injection onto a liquid chromatographic column. Separations on a C-18 silica column and a polystyrene-divinylbenzene column are compared, with a better separation seen on the polymeric column. It is found that the most efficient separations are seen at pH 5 with an acetic acid buffer. Separations of metal-DMAP complexes using different organic modifiers are compared. Good separations using acetonitrile and tetrahydrofuran are seen, and an inversion of retention is seen in tetrahydrofuran. Several metal ions can be separated and quantified; separation conditions, linear calibration curve ranges, and detection limits are presented for Fe(III), U(VI), Ga(III), and Cu(II). Interferences due to the presence of other ions in solution are investigated. Finally a method for selectively determining uranium with postcolumn reactor detection using Arsenazo I is presented.
- Research Organization:
- Iowa State Univ., Ames (USA)
- DOE Contract Number:
- W-7405-ENG-82
- OSTI ID:
- 6481064
- Journal Information:
- Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 60:20; ISSN ANCHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
400102 -- Chemical & Spectral Procedures
400105* -- Separation Procedures
ACTINIDES
AZOLES
CHELATES
CHELATING AGENTS
CHROMATOGRAPHY
COMPLEXES
COPPER
DATA
ELEMENTS
EXPERIMENTAL DATA
GALLIUM
HETEROCYCLIC COMPOUNDS
INFORMATION
IRON
LIQUID COLUMN CHROMATOGRAPHY
METALS
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PERFORMANCE
PH VALUE
PYRAZOLES
PYRAZOLINES
SEPARATION PROCESSES
TRANSITION ELEMENTS
URANIUM