Triangulation of proteins in the 30 S ribosomal subunit of Escherchia coli

Journal Article · Sat Jan 01 04:00:00 EST 1977 · J. Mol. Biol.; (United States)

DOI:https://doi.org/10.1016/S0022-2836(77)80139-3· OSTI ID:6478331

Moore, P B ^[1]; Langer, J A; Schoenborn, B P; Engelman, D M

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1. Yale Univ., New Haven, CT

Thermal neutron radiation has been used for solution scattering experiments on 30 S ribosomal subunits in which specific proteins have been inserted in deuterated form by reconstitution. The data enable us to estimate the distances separating the centers of mass of six protein pairs S3--S4 (61 A), S3--S5 (55 A), S3--S8 (77 A), S4--S7 (86 A), S4--S8 (62 A) and S5--S6 (96 A). Three triangular relationships involving S3, S4, S5, S7 and S8 have been established. Transformation of the data by Patterson inversion gives, for each protein pair, the distribution of lengths of all possible vectors between the two proteins. Model calculations show that the form of the distribution is a function of the shape and orientation of the proteins. The data support the view that many of these proteins are elongated.

• OSTI ID: 6478331
• Journal Information: J. Mol. Biol.; (United States), Journal Name: J. Mol. Biol.; (United States) Vol. 112; ISSN JMOBA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BARYONS
BIOCHEMICAL REACTION KINETICS
CELL CONSTITUENTS
ELEMENTARY PARTICLES
ESCHERICHIA COLI
FERMIONS
HADRONS
KINETICS
MATHEMATICAL MODELS
MICROORGANISMS
NEUTRONS
NUCLEONS
ORGANIC COMPOUNDS
ORGANOIDS
PROTEINS
REACTION KINETICS
RIBOSOMES
SCATTERING
THERMAL NEUTRONS