Neutrophil-mediated protection of cultured human vascular endothelial cells from damage by growing Candida albicans hyphae

Journal Article · Fri May 01 00:00:00 EDT 1987 · Blood; (United States)

OSTI ID:6478128

Edwards, Jr, J E; Rotrosen, D; Fontaine, J W; Haudenschild, C C; Diamond, R D

Interactions were studied between human neutrophils and cultured human umbilical vein endothelial cells invaded by Candida albicans. In the absence of neutrophils, progressive Candida germination and hyphal growth extensively damaged endothelial cell monolayers over a period of 4 to 6 hours, as determined both by morphological changes and release of /sup 51/Cr from radiolabeled endothelial cells. Monolayers were completely destroyed and replaced by hyphae after 18 hours of incubation. In contrast, when added 2 hours after the monolayers had been infected with Candida, neutrophils selectively migrated toward and attached to hyphae at points of hyphal penetration into individual endothelial cells (observed by time-lapse video-microscopy). Attached neutrophils spread over hyphal surfaces both within and beneath the endothelial cells; neutrophil recruitment to initial sites of leukocyte-Candida-endothelial cell interactions continued throughout the first 60 minutes of observation. Neutrophil spreading and stasis were observed only along Candida hyphae and at sites of Candida-endothelial cell interactions. These events resulted in 58.0% killing of Candida at 2 hours and subsequent clearance of Candida from endothelial cell monolayers, as determined by microcolony counts and morphological observation. On introduction of additional neutrophils to yield higher ratios of neutrophils to endothelial cells (10 neutrophils:1 endothelial cell), neutrophil migration toward hyphal elements continued. Despite retraction or displacement of occasional endothelial cells by invading Candida and neutrophils, most endothelial cells remained intact, viable, and motile as verified both by morphological observations and measurement of /sup 51/Cr release from radiolabeled monolayers.

• Research Organization: Harbor-UCLA Medical Center, Torrance
• OSTI ID: 6478128
• Journal Information: Blood; (United States), Vol. 5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CANDIDA
CELL KILLING
INFECTIVITY
NEUTROPHILS
BIOLOGICAL FUNCTIONS
BIOLOGICAL LOCALIZATION
CELL CULTURES
CHROMIUM 51
ENDOTHELIUM
ANIMAL TISSUES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CHROMIUM ISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
FUNCTIONS
FUNGI
INTERMEDIATE MASS NUCLEI
ISOTOPES
LEUKOCYTES
MATERIALS
MICROORGANISMS
NUCLEI
PLANTS
RADIOISOTOPES
TISSUES
YEASTS
550301* - Cytology- Tracer Techniques