Disposition of human fibrinopeptide A in normal and nephrectomized rabbits

Journal Article · Sat Oct 01 04:00:00 EDT 1983 · Thromb. Res.; (United States)

DOI:https://doi.org/10.1016/0049-3848(83)90149-4· OSTI ID:6472326

Harenberg, J; Stehle, G; Waibel, S; Hermann, H J; Eisenhut, M; Zimmermann, R

The distribution, elimination, and metabolism of human fibrinopeptide A (FPA) were studied in normal and nephrectomized rabbits. The activity of /sup 125/I-labeled desamino-tyrosyl human FPA (DAT-FPA) was followed over 4 hours after i.v. administration. Results show that in normal rabbits (n . 10) DAT-FPA is eliminated from plasma in four phases with half-lives of 30 sec, 3.5 min, 15 min, and 90 min. The distribution of /sup 123/I-labeled DAT-FPA in plasma was determined in 15 control rabbits with scintigraphy over 2 hours. DAT-FPA was distributed primarily in the cardiovascular system, liver, and kidneys. In some animals minimal radioactivity was detected over the gall bladder. Radioactivity accumulated rapidly in the urinary bladder, approximately 50% being recorded after 15 min and 90% after 120 min. In the heart area radioactivity decreased with half-lives of 25 sec, 7.5 min, 25 min, and 180 min. Nephrectomized rabbits had similar initial fast distribution of DAT-FPA after administration of /sup 125/I-labeled (n . 10) and /sup 123/I-labeled peptide (n . 10). The estimated half-life of the slow component was in the order of several hours. The results of the scintigraphic and gel chromatographic studies show that FPA is primarily excreted in the urine. Previously reported half-lives of FPA reflect distribution rather than steady state conditions.

• Research Organization: Univ. of Heidelberg, West Germany
• OSTI ID: 6472326
• Journal Information: Thromb. Res.; (United States), Journal Name: Thromb. Res.; (United States) Vol. 32:1; ISSN THBRA
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BILIARY TRACT
BIOLOGICAL HALF-LIFE
BIOLOGICAL MATERIALS
BLADDER
BLOOD
BLOOD COAGULATION FACTORS
BLOOD PLASMA
BODY
BODY FLUIDS
CARDIOVASCULAR SYSTEM
CHROMATOGRAPHY
COAGULANTS
COUNTING TECHNIQUES
DAYS LIVING RADIOISOTOPES
DIAGNOSTIC TECHNIQUES
DIGESTIVE SYSTEM
DISTRIBUTION
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
FIBRINOGEN
GLANDS
GLOBULINS
HEART
HEMATOLOGIC AGENTS
HEMOSTATICS
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 123
IODINE 125
IODINE ISOTOPES
ISOTOPES
KIDNEYS
KINETICS
LABELLED COMPOUNDS
LIVER
MAMMALS
MAN
MATERIALS
MEDICINE
METABOLISM
NEPHRECTOMY
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PEPTIDES
PRIMATES
PROTEINS
RABBITS
RADIOISOTOPE SCANNING
RADIOISOTOPES
SCINTISCANNING
SEPARATION PROCESSES
SURGERY
TISSUE DISTRIBUTION
URINARY TRACT
VERTEBRATES