Skip to main content
OSTI.GOVU.S. Department of Energy Office of Scientific and Technical Information
U.S. Department of Energy
Office of Scientific and Technical Information
 

Human leukocytic pyrogen: purification and development of a radioimmunoassay. [/sup 125/I tracer technique]

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
; ;
Leukocytic pyrogen is a small endogenous protein that mediates fever. Because of the limitations of bioassays, circulating leukocytic pyrogen has not been demonstrated during fever in humans. The pyrogen was produced in vitro after phagocytosis of staphylococci by blood monocytes. Antibody against the pyrogen was obtained from rabbits immunized with leukocytic pyrogen and the antiserum was purified by solid-phase immunoadsorbants. Purified antibody to the pyrogen was attached to activated Sepharose 4B and used in conjunction wtih gel filtration to purify the pyrogen. The pyrogen was labeled with /sup 125/I and further purified by gel filtration and ion-exchange chromatography. The final preparation of /sup 125/I-labeled pyrogen demonstrated a homogeneous band during isoelectric focusing and other separation procedures. With antibody to pyrogen attached to Sepharose, less than 0.1 of a rabbit pyrogenic dose of human leukocytic pyrogen inhibited the binding of /sup 125/I-labeled pyrogen to this immunoadsorbant, and this inhibition was not affected by the presence of human serum. Thus, a radioimmunoassay for human leukocytic pyrogen has been developed that may be used to detect circulating pyrogen during fever in humans.
Research Organization:
National Inst. of Health, Bethesda, MD
OSTI ID:
6471423
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 74:10; ISSN PNASA
Country of Publication:
United States
Language:
English

Similar Records

Use of an anti-human leukocyte interferon monoclonal antibody for the purification and radioimmunoassay of human alpha interferon
Journal Article · Wed Sep 01 00:00:00 EDT 1982 · Infect. Immun.; (United States) · OSTI ID:6564607
Use of immunoaffinity chromatography for purification of /sup 125/I-labeled human prolactin
Journal Article · Mon Jan 31 23:00:00 EST 1983 · Clin. Chem. (Winston-Salem, N.C.); (United States) · OSTI ID:6191737
Production of specific antisera for radioimmunoassay of human luteinizing hormone (LH) in the presence of human chorionic gonadotropin (hCG). [/sup 125/I]
Journal Article · Wed Sep 01 00:00:00 EDT 1976 · J. Clin. Endocrinol. Metab.; (United States) · OSTI ID:7332391

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
PURIFICATION
PYROGENS
RABBITS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOISOTOPES
TRACER TECHNIQUES
VERTEBRATES