Erythropoiesis in the aged mouse. II. Response to stimulation in vitro

Journal Article · Sun Apr 01 04:00:00 EST 1984 · J. Lab. Clin. Med.; (United States)

OSTI ID:6471057

Udupa, K B; Lipschitz, D A

In this study, in vitro evidence is presented that erythropoietic precursors in aged mice respond less to stimulation by erythropoietin than do precursors in young mice. The effect of age on proliferation of differentiated erythroid cells from the marrow of young and old mice was examined in liquid culture to which increasing concentrations of erythropoietin were added. Cellular proliferation was measured indirectly by /sup 59/Fe incorporation into heme and directly as tritiated thymidine incorporation into DNA. The number of normoblasts remaining in culture with and without the addition of erythropoietin was also measured. In each case, cellular proliferation was significantly lower in marrow in old than in young mice. In contrast, CFU-E colonies cultured with increasing doses of erythropoietin were similar in young and old animals. These findings indicate that aging causes a reduction in the proliferative response of differentiated erythroid cells. Failure of these cells to respond to stimulation is the likely mechanism for the reduced erythropoietic proliferative capacity found in aged animals.

• Research Organization: Little Rock V.A. Medical Center, AR
• OSTI ID: 6471057
• Journal Information: J. Lab. Clin. Med.; (United States), Journal Name: J. Lab. Clin. Med.; (United States) Vol. 103:4; ISSN JLCMA
• Country of Publication: United States
• Language: English

Related Subjects

551001* -- Physiological Systems-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANIMALS
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOSYNTHESIS
BONE MARROW CELLS
CARBOXYLIC ACIDS
CELL CULTURES
CELL PROLIFERATION
CFU
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DNA
ERYTHROPOIETIN
EVEN-ODD NUCLEI
GROWTH
HEME
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HYDROGEN ISOTOPES
IN VITRO
INTERMEDIATE MASS NUCLEI
IRON 59
IRON ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LIFE CYCLE
LIGHT NUCLEI
MAMMALS
MICE
NUCLEI
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PIGMENTS
PORPHYRINS
PYRIMIDINES
RADIOISOTOPES
RESPONSE MODIFYING FACTORS
RIBOSIDES
RODENTS
SOMATIC CELLS
SYNTHESIS
THYMIDINE
TRACER TECHNIQUES
TRITIUM
UPTAKE
VERTEBRATES
YEARS LIVING RADIOISOTOPES