Ethanologenic enzymes of Zymomonas mobilis: Progress report

Technical Report · Wed Feb 01 04:00:00 EST 1989

OSTI ID:6469239

Ingram, L O

In this study, we have proposed to investigate the regulatory mechanisms which permit the high level expression of the ethanologenic enzymes from Zymomonas mobilis (PDC, ADHI, ADHII). This research is continuing essentially as proposed in the original grant except that the scope is being expanded to include the glycolytic enzymes which are also highly expressed. Several enzymes which are expressed only at moderate levels are being examined for comparison (tryptophan biosynthesis, acid phosphatase). Studies of highly expressed genes involve enzyme purification and the production of antibodies, investigations of the effects of growth conditions on expression, cloning and characterization of structural genes, construction of hybrid genes, mutation of alcohol dehydrogenases, and investigation of transcriptional and translational regulation. In addition, we are investigating the feasibility of replacing the NAD regeneration systems of other bacteria with an artificial operon containing the Z. mobilis genes (PDC and ADHII) for the production of ethanol, the ''PET'' operon. 30 refs.

• Research Organization: Florida Univ., Gainesville (USA). Dept. of Microbiology and Cell Science
• DOE Contract Number: FG05-86ER13574
• OSTI ID: 6469239
• Report Number(s): DOE/ER/13574-3; ON: DE89008087
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
090222 -- Alcohol Fuels-- Preparation from Wastes or Biomass-- (1976-1989)
550200* -- Biochemistry
550400 -- Genetics
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
BACTERIA
CARBON-CARBON LYASES
CARBOXY-LYASES
DECARBOXYLASES
DNA SEQUENCING
DOCUMENT TYPES
ENZYME ACTIVITY
ENZYMES
GENE REGULATION
GENE REPRESSORS
LYASES
MICROORGANISMS
MOLECULAR BIOLOGY
MOLECULAR STRUCTURE
NUCLEOPROTEINS
ORGANIC COMPOUNDS
PROGRESS REPORT
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
ZYMOMONAS MOBILIS