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Title: Serological responses in chimpanzees inoculated with human immunodeficiency virus glycoprotein (gp120) subunit vaccine

Abstract

The major envelope glycoprotein of a human immunodeficiency virus (HIV) has been purified and was utilized as a prototype vaccine in chimpanzees. The 120,000-dalton glycoprotein (gp120) was purified from membranes of human T-lymphotropic virus (HTLV)-IIIB-infected cells and the final preparation contained low levels to no detectable HTLV-IIIB core antigen (p24) and low levels of endotoxin. Chimpanzees inoculated with gp120 responded by developing antibodies that precipitated radiolabeled gp120 and neutralized in vitro infection of HTLV-IIIB. Antibodies to HTLV-IIIB p24 were not detected in the gp120-immunized chimpanzees. Peripheral blood leukocytes from the vaccinated animals were examined for T4/sup +/ and T8/sup +/ cells, and no decrease in the T4/T8 ratio was found, indicating that immunization with a ligand (gp120) that binds to T4 has not detectable adverse effect on the population of T4/sup +/ cells. The only current animal model that can be reproducibly infected with HIV is the chimpanzee. Immunization of chimpanzees with HIV proteins will provide an experimental system for testing the effectiveness of prototype vaccines for preventing HIV infection in vivo.

Authors:
; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Program Resources, Inc., Frederick, MD (USA)
OSTI Identifier:
6466492
Resource Type:
Journal Article
Journal Name:
Proc. Natl. Acad. Sci. U.S.A.; (United States)
Additional Journal Information:
Journal Volume: 84:23
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; AIDS VIRUS; IMMUNOLOGY; GLUCOPROTEINS; AUTORADIOGRAPHY; RADIOIMMUNOASSAY; AIDS; BLOOD SERUM; ELECTROPHORESIS; IODINE 125; MOLECULAR STRUCTURE; PRECIPITATION; PRIMATES; PURIFICATION; VACCINES; ANIMALS; BETA DECAY RADIOISOTOPES; CARBOHYDRATES; DAYS LIVING RADIOISOTOPES; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; GLYCOPROTEINS; HEMIC DISEASES; IMMUNE SYSTEM DISEASES; IMMUNOASSAY; INFECTIOUS DISEASES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; MICROORGANISMS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PARASITES; PROTEINS; RADIOASSAY; RADIOIMMUNOLOGY; RADIOISOTOPES; SACCHARIDES; SEPARATION PROCESSES; TRACER TECHNIQUES; VERTEBRATES; VIRAL DISEASES; VIRUSES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics

Citation Formats

Arthur, L O, Pyle, S W, Nara, P L, Bess, Jr, J W, Gonda, M A, Kelliher, J C, Gilden, R V, Robey, W G, Bolognesi, D P, and Gallo, R C. Serological responses in chimpanzees inoculated with human immunodeficiency virus glycoprotein (gp120) subunit vaccine. United States: N. p., 1987. Web. doi:10.1073/pnas.84.23.8583.
Arthur, L O, Pyle, S W, Nara, P L, Bess, Jr, J W, Gonda, M A, Kelliher, J C, Gilden, R V, Robey, W G, Bolognesi, D P, & Gallo, R C. Serological responses in chimpanzees inoculated with human immunodeficiency virus glycoprotein (gp120) subunit vaccine. United States. https://doi.org/10.1073/pnas.84.23.8583
Arthur, L O, Pyle, S W, Nara, P L, Bess, Jr, J W, Gonda, M A, Kelliher, J C, Gilden, R V, Robey, W G, Bolognesi, D P, and Gallo, R C. 1987. "Serological responses in chimpanzees inoculated with human immunodeficiency virus glycoprotein (gp120) subunit vaccine". United States. https://doi.org/10.1073/pnas.84.23.8583.
@article{osti_6466492,
title = {Serological responses in chimpanzees inoculated with human immunodeficiency virus glycoprotein (gp120) subunit vaccine},
author = {Arthur, L O and Pyle, S W and Nara, P L and Bess, Jr, J W and Gonda, M A and Kelliher, J C and Gilden, R V and Robey, W G and Bolognesi, D P and Gallo, R C},
abstractNote = {The major envelope glycoprotein of a human immunodeficiency virus (HIV) has been purified and was utilized as a prototype vaccine in chimpanzees. The 120,000-dalton glycoprotein (gp120) was purified from membranes of human T-lymphotropic virus (HTLV)-IIIB-infected cells and the final preparation contained low levels to no detectable HTLV-IIIB core antigen (p24) and low levels of endotoxin. Chimpanzees inoculated with gp120 responded by developing antibodies that precipitated radiolabeled gp120 and neutralized in vitro infection of HTLV-IIIB. Antibodies to HTLV-IIIB p24 were not detected in the gp120-immunized chimpanzees. Peripheral blood leukocytes from the vaccinated animals were examined for T4/sup +/ and T8/sup +/ cells, and no decrease in the T4/T8 ratio was found, indicating that immunization with a ligand (gp120) that binds to T4 has not detectable adverse effect on the population of T4/sup +/ cells. The only current animal model that can be reproducibly infected with HIV is the chimpanzee. Immunization of chimpanzees with HIV proteins will provide an experimental system for testing the effectiveness of prototype vaccines for preventing HIV infection in vivo.},
doi = {10.1073/pnas.84.23.8583},
url = {https://www.osti.gov/biblio/6466492}, journal = {Proc. Natl. Acad. Sci. U.S.A.; (United States)},
number = ,
volume = 84:23,
place = {United States},
year = {Tue Dec 01 00:00:00 EST 1987},
month = {Tue Dec 01 00:00:00 EST 1987}
}