Lactoperoxidase catalyzed radioiodination of cell surface immunoglobulin: incorporated radioactivity may not reflect relative cell surface Ig density. [/sup 125/I]

Journal Article · Thu Feb 01 00:00:00 EST 1979 · J. Immunol.; (United States)

OSTI ID:6466127

Wilder, R L; Yuen, C C; Mage, R G

Rabbit and mouse splenic lymphocytes were radioiodinated by the lactoperoxidase technique, extracted with non-ionic detergent, immunoprecipitated with high titered rabbit anti-kappa antisera, and compared by SDS-PAGE. Mouse sIg peaks were reproducibly larger in size than rabbit sIg peaks (often greater than 10 times). Neither differences in incorporation of label into the rabbit cell surface, nor differences in average sIg density explain this result. Total TCA-precipitable radioactivity was similar in each species. Estimation of the relative amounts of sIg in the mouse and rabbit showed similar average sIg densities. Differences in detergent solubility, proteolytic lability, or antisera used also do not adequately account for this difference. Thus, these data indicate that radioactivity incorporated after lactoperoxidase catalyzed cell surface radioiodination may not reflect cell surface Ig density. Conclusions about cell surface density based upon relative incorporation of radioactivity should be confirmed by other approaches.

• Research Organization: National Inst. of Health, Bethesda, Maryland
• OSTI ID: 6466127
• Journal Information: J. Immunol.; (United States), Vol. 122:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
IMMUNOGLOBULINS
METABOLISM
LYMPHOCYTES
RADIONUCLIDE KINETICS
PEROXIDASES
DENSITY
IMMUNE SERUMS
IODINE 125
MICE
RABBITS
RADIOACTIVITY
SOLUBILITY
SURFACES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
ENZYMES
GLOBULINS
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPES
LEUKOCYTES
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDOREDUCTASES
PHYSICAL PROPERTIES
PORPHYRINS
PROTEINS
RADIOISOTOPES
RODENTS
SOMATIC CELLS
VERTEBRATES
560170* - Radiation Effects- Nuclide Kinetics & Toxicology- (-1987)