Nuclear estrogen receptor molecular heterogeneity in the mouse uterus

Journal Article · Fri Jan 01 00:00:00 EST 1988 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.85.1.69· OSTI ID:6464683

Golding, T S; Korach, K S

Holomeric estrogen receptor (ER) prepared from ovariectomized mouse uteri displays heterogeneous electrophoretic mobility when analyzed by NaDodSO/sub 4//PAGE. ER derived from nuclei (ER/sub n/) appears as a closely spaced doublet having apparent molecular masses of 66.4 and 65 kDa, while ER from the cytosolic compartment (ER/sub c/) has a single band of 65 kDa. Both partially purified ER/sub c/ and the 8S form of unactivated ER/sub c/ show only the 65-kDa band. The appearance of the ER/sub n/ doublet is hormonally inducible, and the relative proportions of the two doublet bands are influenced by the type of hormone treatment, with weakly estrogenic compounds yielding the lower band as predominant while potent estrogens increase the proportion of the upper band. Steroid binding of the ER/sub n/ doublet was determined by (/sup 3/H)tamoxifen aziridine affinity labeling of both the 66.4- and the 65-kDa peptides; binding to the 65-kDa peptide was predominant. The ER/sub n/ doublet displays a time dependency after estrogen administration with maximal amounts occurring in a bimodal fashion at 1 and 8 hr.

• Research Organization: National Institutes of Health, Research Triangle Park, NC (USA)
• OSTI ID: 6464683
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 85:1
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
RECEPTORS
MOLECULAR STRUCTURE
TAMOXIFEN
RADIORECEPTOR ASSAY
AUTORADIOGRAPHY
ELECTROPHORESIS
ESTROGENS
IODINE 125
MICE
THIN-LAYER CHROMATOGRAPHY
TRITIUM COMPOUNDS
UTERUS
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
FEMALE GENITALS
HORMONES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
RODENTS
SEPARATION PROCESSES
STEROID HORMONES
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques