Cytochrome oxidase heme-protein dynamics: a transient Raman study of carbon monoxide photolysis from cytochrome a

Journal Article · Wed Sep 19 00:00:00 EDT 1984 · J. Am. Chem. Soc.; (United States)

DOI:https://doi.org/10.1021/ja00331a059· OSTI ID:6462637

Findsen, E W; Ondrias, M R

Data are reported on results of initial efforts to probe the mechanism of cytochrome oxidase function by utilizing time-resolved resonance Raman spectroscopy. Preparation of the reduced beef-heart cytochrome oxidase sample and cytochrome oxidase-CO sample is described. At the laser powers and concentrations employed, the reduced cytochrome oxidase-CO sample underwent almost complete photolysis during the laser pulse. Principal conclusions drawn from spectral analysis are that time-resolved resonance Raman investigation of the transient heme species generated by ligand photolysis is a viable technique for the study of heme-ligand dynamics in proteins other than hemoglobin. A transient proximal geometry leading to a strengthened iron-histidine bond is present in these. The interplay of porphyrin core size, pi electron density, and Fe-His bonding as modulated by heme-protein dynamics is different for the ligand binding sites of hemoglobin and cytochrome oxidase. 17 references, 1 figure.

• Research Organization: Univ. of New Mexico, Albuquerque
• OSTI ID: 6462637
• Journal Information: J. Am. Chem. Soc.; (United States), Journal Name: J. Am. Chem. Soc.; (United States) Vol. 106:19; ISSN JACSA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400301* -- Organic Chemistry-- Chemical & Physicochemical Properties-- (-1987)
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
AZOLES
BODY
CARBON COMPOUNDS
CARBON MONOXIDE
CARBON OXIDES
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CHALCOGENIDES
CHEMICAL REACTIONS
COMPLEXES
CYTOCHROME OXIDASE
DATA
DATA ANALYSIS
DECOMPOSITION
ELECTROMAGNETIC RADIATION
ELECTRONIC STRUCTURE
ENERGY LEVELS
ENZYME ACTIVITY
ENZYMES
EXCITED STATES
EXPERIMENTAL DATA
FOOD
HAEM DEHYDROGENASES
HEART
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
HISTIDINE
IMIDAZOLES
INFORMATION
IRON COMPLEXES
LASER RADIATION
LIGANDS
MEAT
MOLECULAR STRUCTURE
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
OXIDES
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHOTOLYSIS
PORPHYRINS
RADIATIONS
RAMAN SPECTRA
REDUCTION
SAMPLE PREPARATION
SPECTRA
STRUCTURAL CHEMICAL ANALYSIS
TIME DEPENDENCE
TRANSITION ELEMENT COMPLEXES