Effect of in vivo ozone exposure on in vitro pulmonary alveolar macrophage mobility. [Rats]
The effect of in vivo O/sub 3/ exposure on the mobility of pulmonary alveolar macrophages (PAM) in vitro was investigated. Eight randomly selected rats were exposed for 4 h. Four rats were exposed to a clean air (sham) atmosphere, and four to an atmosphere containing 1 ppM O/sub 3/. PAM were obtained by lung lavage and placed on gold-colloid coated coverslips. The area cleared of gold particles by migrating PAM after 24, 48, and 72 h was used as an indicator of cell mobility. The number of PAM recoverable by lavage was similar for both groups (2 x 10/sup 5/), but the percentage of macrophages that made tracks was significantly smaller with 95% certainty in the O/sub 3/ group. For sham-exposed and O/sub 3/-exposed groups, the area cleared by the sham-exposed group being about 50% greater during each time period. It was concluded that the in vitro migrational potential of PAM was most likely decreased by in vivo exposure to O/sub 3/.
- Research Organization:
- Univ. of California, Irvine
- OSTI ID:
- 6459168
- Journal Information:
- J. Toxicol. Environ. Health; (United States), Vol. 7:3-4
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
MACROPHAGES
MOBILITY
OZONE
BIOLOGICAL EFFECTS
ACUTE EXPOSURE
IN VITRO
IN VIVO
LAVAGE
LUNGS
PARTICLE TRACKS
RATS
ANIMAL CELLS
ANIMALS
BODY
CONNECTIVE TISSUE CELLS
MAMMALS
ORGANS
PHAGOCYTES
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)