Fermentation of cellodextrins to ethanol using mixed-culture fermentations

Journal Article · Mon Jul 01 04:00:00 EDT 1985 · Biotechnol. Bioeng.; (United States)

DOI:https://doi.org/10.1002/bit.260270726· OSTI ID:6451620

Freer, S N; Wing, R E

The potential for enhancing ethanol production from cellodextrins by employing mixed-culture (Candida wickerhamii-Saccharomyces cerevisiae) fermentations was investigated. Initially, ethanol production was monitored in fermentation medium containing 50 g/l glucose plus 45 g/l cellobiose. Inoculum levels and times of inoculum addition were varied. Of the conditions tested, the most rapid rates of ethanol formation occurred in fermentations in which either C. wickerhamii and S. cerevisiae were coinoculated at a ratio of 57 : 1 cell/ml or in fermentations in which a 10-fold-greater S. cerevisiae inoculum was added to a pure culture C. wickerhamii fermentation after one day incubation. These conditions were used to attempt to enhance fermentations in which cellodextrins produced by trifluoroacetic acid hydrolysis of cellulose served as the sole carbon source. Cellodextrins that were not further purified after cellulose hydrolysis contained compounds that were slightly inhibitory to C. wickerhamii. In this case the mixed-culture fermentations produced 12-45% more ethanol than a pure culture C. wickerhamii fermentation. However, if the substrate was treated with Darco G-60 charcoal, the toxic materials were apparently removed and the pure culture C. wickerhamii fermentations performed as well as the mixed-culture fermentations.

• OSTI ID: 6451620
• Journal Information: Biotechnol. Bioeng.; (United States), Journal Name: Biotechnol. Bioeng.; (United States) Vol. 27:7; ISSN BIBIA
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ACID HYDROLYSIS
ALCOHOLS
ALDEHYDES
BIOCONVERSION
BIOSYNTHESIS
CANDIDA
CARBOHYDRATES
CELLOBIOSE
CHEMICAL REACTIONS
CULTURE MEDIA
DECOMPOSITION
DEXTRIN
DISACCHARIDES
DISPERSIONS
ETHANOL
FERMENTATION
FUNGI
GLUCOSE
HEXOSES
HYDROLYSIS
HYDROXY COMPOUNDS
LYSIS
MICROORGANISMS
MIXTURES
MONOSACCHARIDES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PERFORMANCE
PLANTS
POLYSACCHARIDES
SACCHARIDES
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
SOLVOLYSIS
SYNTHESIS
YEASTS