Isolation and quantitation of. beta. -D-glucoNyranosyl abscisate from leaves of Xanthium and spinach

Journal Article · Thu Jul 01 00:00:00 EDT 1982 · Plant Physiol.; (United States)

OSTI ID:6450793

Boyer, G L; Zeevaart, J A.D.

Xanthium leaves are known to contain a high level of alkali-hydrolyzable conjugated abscisic acid. This abscisic acid conjugate has been isolated and identified by mass spectrometry, nuclear magnetic resonance, and chemical and enzymic degradation techniques, as the glucosyl ester of abscisic acid, ..beta..-D-glucopyranosyl abscisate. The glucosyl ester of abscisic acid was the only abscisic acid conjugate found in Xanthium leaves. It was also isolated from spinach leaves. An insignificant amount of the glucosyl ester of abscisic acid partitioned into diethyl ether, whereas 12% partitioned into ethyl acetate. Consequently, removal of absicsic acid by partitioning with ethyl acetate will result in considerable losses of the glucosyl ester of abscisic acid from the aqueous phase. Diethyl ether is, therefore, recommended for separation of abscisic acid and the glucosyl ester of abscisic acid by solvent partitioning. A method for quantitation of the glucosyl ester of abscisic acid as the tetraacetate derivative by gas-liquid chromatography with an electron capture detector was developed. The level of ..beta..-D-glycopyranosyl abscisate in Xanthium leaves increased from 3.6 nanomoles per gram fresh weight in turgid leaves to 22.9 nanomoles in leaves from plants subjected to seven wilting-recovery cycles. ..beta..-D-glycopyranosyl abscisate in Xanthium leaves may be a stable end product of abscisic acid metabolism.

• Research Organization: Michigan State Univ., East Lansing
• DOE Contract Number: AC02-76ER01338
• OSTI ID: 6450793
• Journal Information: Plant Physiol.; (United States), Vol. 70:1
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
ORGANIC ACIDS
QUANTITATIVE CHEMICAL ANALYSIS
SOLVENT EXTRACTION
ELECTRON-CAPTURE DETECTORS
ESTERS
GAS CHROMATOGRAPHY
LEAVES
PLANTS
SOLVENTS
CHEMICAL ANALYSIS
CHROMATOGRAPHY
EXTRACTION
MEASURING INSTRUMENTS
ORGANIC COMPOUNDS
RADIOMETRIC GAGES
SEPARATION PROCESSES
400102* - Chemical & Spectral Procedures
400105 - Separation Procedures