Effects of phenol on ATPase activities in crude gill homogenates of rainbow trout (Salmo gairdneri Richardson)
The ATPase specific activities from crude gill homogenates of rainbow trout were lower than those from microsomal preparations reported in the literature. Sodium pump activity (ouabain sensitive NaK-ATPase) was demonstrable at 37/sup 0/C. An ouabain insensitive NaK-ATPase was demonstrable at temperatures below 30/sup 0/C and may represent a Na-ATPase activity reported by others. Energy of activation at 25/sup 0/C for total NaK-ATPase ws 10,500 cal.mole/sup -1/. Mg-baseline activity had an energy of activation at 25/sup 0/C of 15,600 cal.mole/sup -1/. Mg-baseline activity was thermally labile at temperatures in excess of 30/sup 0/C. Concentrations of Mg/sup +2/ in excess of 5 mM appeared to inhibit total NaK-ATPase activity. At 37/sup 0/C, Na/sup +/ and K/sup +/ exerted little, if any, stimulatory effect on ATPase activities, in spite of the fact that 37/sup 0/C was the only temperature at which sodium pump activity was demonstrable. MS-222 failed to produce any discernible changes in any of the demonstrable ATPase activities in crude gill homogenates. Total NaK-ATPase activities were more sensitive than Mg-baseline activities to in vitro inhibition by phenol. Concentrations of phenol which produce 50% inhibition in total NaK-ATPase produced only 35% inhibition in Mg-baseline activity. The nature of in vitro inhibition was uncompetitive. Sodium pump activity was unaffected by phenol at concentrations as high as 25 mM. An effort was made to demonstrate an in vivo effects of phenol on rainbow trout gill ATPase activites. An infestation of a parasite (Gyrodactilus) on the experimental fish precludes any definative assessment of in vivo effects.
- Research Organization:
- Battelle Pacific Northwest Labs., Richland, WA (USA)
- DOE Contract Number:
- EY-76-C-06-1830
- OSTI ID:
- 6450402
- Report Number(s):
- PNL-2697
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560305* -- Chemicals Metabolism & Toxicology-- Vertebrates-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL COMPOUNDS
ALKALINE EARTH METAL COMPOUNDS
ANIMALS
AQUATIC ORGANISMS
AROMATICS
ATP-ASE
BIOLOGICAL EFFECTS
BODY
CATIONS
CHARGED PARTICLES
COMPILED DATA
DATA FORMS
ENZYME ACTIVITY
ENZYMES
ESTERASES
ESTERS
FISHES
GILLS
HOMOGENATES
HYDROLASES
HYDROXY COMPOUNDS
IN VITRO
INHIBITION
IONS
ISOLATED VALUES
MAGNESIUM COMPOUNDS
MEDIUM TEMPERATURE
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PHENOL
PHENOLS
PHOSPHATASES
POTASSIUM COMPOUNDS
RESPIRATORY SYSTEM
SODIUM COMPOUNDS
SULFONIC ACID ESTERS
TEMPERATURE DEPENDENCE
TISSUES
TOXICITY
TROUT
VERTEBRATES