Use of repetitive sequences and the polymerase chain reaction technique to classify genetically related Bradyrhizobium japonicum serocluster 123 strains

Journal Article · Tue Jun 01 00:00:00 EDT 1993 · Applied and Environmental Microbiology; (United States)

OSTI ID:6449346

Judd, A K; Sadowsky, M J ^[1]; Schneider, M; Bruijn, F.J. de ^[2]

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1. Univ. of Minnesota, St. Paul (United States) MSU-DOE Plant Research Lab., East Lansing, MI (United States)
2. Univ. of Minnesota, St. Paul (United States) Michigan State Univ., East Lansing (United States)

Bacteria of the genus Bradyrhizobium are slow-growing, gram-negative, heterotrophic and have the ability to form root nodules on several leguminous plants. In the upper midwest USA, members of B. japonicum serocluster 123 are the dominant indigenous competitors for the nodulation of soybeans. However, the current serological divisions fail to adequately reflect the genetic and phenotypic diversity among member isolates. This article describes the use of REP and ERIC PCR fingerprinting and RFLP analysis using a hyperreiterated DNA probe to classiby serologically related and genetically similar B. japonicum serocluster 123 strains. In addition the comparison of 15 serologically distinct B. japonicum and Bradyrhizobia spp. strains by using REP and ERIC PCR fingerprint analysis is presented. 25 refs., 8 figs., 1 tab.

• OSTI ID: 6449346
• Journal Information: Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 59:6; ISSN AEMIDF; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BIOLOGICAL VARIABILITY
CLASSIFICATION
DEVELOPED COUNTRIES
DNA
FOOD
GENETIC VARIABILITY
LEGUMINOSAE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
MICROORGANISMS
NITROGEN FIXATION
NORTH AMERICA
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLANTS
SOYBEANS
STRAINS
USA
VEGETABLES