Alteration of diphenamid metabolism by ozone

Journal Article · Fri Jan 01 04:00:00 EST 1971 · Weed Sci Soc Am; (United States)

OSTI ID:6447016

Hodgson, R H; Fargo, N D

Tomato seedlings were grown under controlled environmental conditions. Plants were fumigated with 18-20 pphm ozone for 17-19 hr before their roots were exposed to carbonyl-/sup 14/C-labeled diphenamid. Fumigation with ozone was continued during the 24 to 48 hr diphenamid treatment. After exposure, the tissue was extracted in chloroform-water-methanol. Typical symptoms of ozone damage, including reddish interveinal lesions had begun to develop at the time of plant harvest. Plants absorbed 50 to 85% of the radioactivity administered, most of which was found in the leaves. Fumigated plants absorbed 70 to 100% as much diphenamid as did controls. The chloroform-soluble fractions of extracts from both fumigated and non-fumigated plants contained, in addition to diphenamid, N-methyl-2,2-diphenylacetamide (MDA), 2,2-diphenylacetamide(DA), and at least one unidentified compound. The level of diphenamid was 41% lower in treated tissue than in controls, while MDA and DA were enhanced 35 and 30%, respectively. Water soluble extracts of both treated and control plants were chromatographed in n-butanol:acetic acid:water, 120:30:50. Both contained activity at Rf = 0.67, which appears to be a glucoside hydrolizable to MDA and glucose. In addition, fumigated tissue contained activity at Rf 0.48, which can be hydrolized to a material cochromatographing with MDA and moieties positive to sugar reagents. Further characterization of this new metabolite is in progress.

• Research Organization: Dept. of Agriculture, Fargo, ND
• OSTI ID: 6447016
• Journal Information: Weed Sci Soc Am; (United States), Journal Name: Weed Sci Soc Am; (United States)
• Country of Publication: United States
• Language: English

Related Subjects

500200 -- Environment
Atmospheric-- Chemicals Monitoring & Transport-- (-1989)
54 ENVIRONMENTAL SCIENCES
560303* -- Chemicals Metabolism & Toxicology-- Plants-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMIDES
BIOLOGICAL EFFECTS
CARBON 14 COMPOUNDS
FOOD
INJURIES
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
METABOLISM
METABOLITES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OZONE
PLANTS
SEEDLINGS
TOMATOES
TRACER TECHNIQUES
UPTAKE
VEGETABLES