Production of radiolabeled monoclonal antibody conjugates by photoaffinity labeling

Technical Report · Fri Jun 01 00:00:00 EDT 1990

DOI:https://doi.org/10.2172/6445745· OSTI ID:6445745

Volkert, W A; Ketring, A R; Kuntz, R R; Holmes, R A; Mitchell, E P ^[1]; Feldbush, T L ^[2]

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1. Missouri Univ., Columbia, MO (USA)
2. Harry S. Truman Memorial Veterans Hospital, Columbia, MO (USA)

This report discusses activities and progress that has occurred since initiation of this project on September 1, 1989. We have synthesized ethyl N,N{prime}-bis(benzoylmercaptoacetyl)-2,3-diaminopropanoate, a ligand to be used as a bifunctional chelating agent (BFCA), to form {sup 186}Re or {sup 188}Re ({sup 186}Re/{sup 188}Re) complexes. {sup 186}Re/{sup 188}Re, in reducing media, reacts with this ligand to form {sup 186}Re/{sup 188}Re-CO{sub 2}DADS chelates that will be used to formulate new radiolabeled photoaffinity labels (RPALs). Initial steps have been taken to synthesize R-As-dithiol compounds. This approach will be used to produce {sup 77}As-RPALs or covalently link {sup 77}As directly to monoclonal antibodies (MAbs). The R group will contain a group that can be used for conjugation reactions. Spectral and photochemical properties of various types of photoaffinity labels (PALs) have been studied. Acrylo-azido compounds and 9-azido acridine have been studied as well as several other photoprobes. The binding characteristics of the azido-based PALs to HSA have been studied and progress has been made on developing techniques for efficiently separating of non-covalently sound PALs. The Nd-YAG laser was purchased and arrived in 1990. It has been assembled and tested and is now operational.

• Research Organization: Missouri Univ., Columbia, MO (USA)
• Sponsoring Organization: DOE/ER
• DOE Contract Number: FG02-89ER60875
• OSTI ID: 6445745
• Report Number(s): DOE/ER/60875-1; ON: DE91000827
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
38 RADIATION CHEMISTRY, RADIOCHEMISTRY, AND NUCLEAR CHEMISTRY
MONOCLONAL ANTIBODIES
LABELLING
RHENIUM COMPLEXES
SYNTHESIS
ACRIDINES
AFFINITY
ARSENIC 77
ARSENIC COMPOUNDS
CHELATES
CHELATING AGENTS
CHEMICAL REACTIONS
LIGANDS
PHOTOCHEMISTRY
PROGRESS REPORT
REACTION INTERMEDIATES
RHENIUM 186
RHENIUM 188
SEPARATION PROCESSES
ANTIBODIES
AROMATICS
ARSENIC ISOTOPES
AZAARENES
AZINES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMISTRY
COMPLEXES
DAYS LIVING RADIOISOTOPES
DOCUMENT TYPES
ELECTRON CAPTURE RADIOISOTOPES
HEAVY NUCLEI
HETEROCYCLIC COMPOUNDS
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-EVEN NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PYRIDINES
RADIOISOTOPES
RHENIUM ISOTOPES
TRANSITION ELEMENT COMPLEXES
550201* - Biochemistry- Tracer Techniques
400703 - Radiochemistry & Nuclear Chemistry- Radioisotope Production