Plasmid-mediated mineralization of naphthalene, phenanthrene, and anthracene

Journal Article · Tue Jun 01 00:00:00 EDT 1993 · Applied and Environmental Microbiology; (United States)

OSTI ID:6444971

Sanseverino, J ^[1]; Applegate, B M; King, J M.H.; Sayler, G S ^[2]

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1. Univ. of Tennessee, Knoxville (United States) IT Corp., Knoxville, TN (United States)
2. Univ. of Tennessee, Knoxville (United States)

The biochemistry and genetics of the naphthalene degradation pathway contained on plasmid NAH7 have been well characterized. However, not much is known about the substrate specificity of the enzymes of nah operons and whether the nah-encoded enzymes are capable of metabolizing higher polyaromatic hydrocarbons. This paper shows that NAH7 and NAH7-like plasmids can mediate metabolism of phenanthrene and anthracene as well as naphthalene. In addition, a mutant blocked in the nahG (salicylate hydroxylase) gene produced unidentified metabolites when it is grown in the presence of phenanthrene and anthracene. This implies that phenanthrene and anthracene are degraded through the nah plasmid-encoded system. 29 refs., 3 figs., 2 tabs.

• OSTI ID: 6444971
• Journal Information: Applied and Environmental Microbiology; (United States), Vol. 59:6; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIODEGRADATION
BIOLOGICAL PATHWAYS
PLASMIDS
POLYCYCLIC AROMATIC HYDROCARBONS
ANTHRACENE
ENZYMES
METABOLISM
MINERALIZATION
NAPHTHALENE
PHENANTHRENE
PSEUDOMONAS
AROMATICS
BACTERIA
CELL CONSTITUENTS
CHEMICAL REACTIONS
CONDENSED AROMATICS
DECOMPOSITION
HYDROCARBONS
MICROORGANISMS
ORGANIC COMPOUNDS
PROTEINS
560300* - Chemicals Metabolism & Toxicology