Plasmid-mediated mineralization of naphthalene, phenanthrene, and anthracene

Journal Article · Tue Jun 01 00:00:00 EDT 1993 · Applied and Environmental Microbiology; (United States)

OSTI ID:6444971

Sanseverino, J ^[1]; Applegate, B M; King, J M.H.; Sayler, G S ^[2]

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1. Univ. of Tennessee, Knoxville (United States) IT Corp., Knoxville, TN (United States)
2. Univ. of Tennessee, Knoxville (United States)

The biochemistry and genetics of the naphthalene degradation pathway contained on plasmid NAH7 have been well characterized. However, not much is known about the substrate specificity of the enzymes of nah operons and whether the nah-encoded enzymes are capable of metabolizing higher polyaromatic hydrocarbons. This paper shows that NAH7 and NAH7-like plasmids can mediate metabolism of phenanthrene and anthracene as well as naphthalene. In addition, a mutant blocked in the nahG (salicylate hydroxylase) gene produced unidentified metabolites when it is grown in the presence of phenanthrene and anthracene. This implies that phenanthrene and anthracene are degraded through the nah plasmid-encoded system. 29 refs., 3 figs., 2 tabs.

• OSTI ID: 6444971
• Journal Information: Applied and Environmental Microbiology; (United States), Journal Name: Applied and Environmental Microbiology; (United States) Vol. 59:6; ISSN AEMIDF; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANTHRACENE
AROMATICS
BACTERIA
BIODEGRADATION
BIOLOGICAL PATHWAYS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CONDENSED AROMATICS
DECOMPOSITION
ENZYMES
HYDROCARBONS
METABOLISM
MICROORGANISMS
MINERALIZATION
NAPHTHALENE
ORGANIC COMPOUNDS
PHENANTHRENE
PLASMIDS
POLYCYCLIC AROMATIC HYDROCARBONS
PROTEINS
PSEUDOMONAS