Localization of the kappa opioid receptor gene to human chromosome band 8q11. 2
- Univ. of Chicago, IL (United States)
Using the cloned mouse kappa opioid receptor cDNA clone as a probe, screened a human genomic library and isolated a clone containing part of the human kappa opioid receptor gene (OPRK1), designated [lambda]hSR4-1. To determine the chromosomal localization of OPRK1, [lambda]hSR4-1 DNA was labeled with biotin by nick-translation in the presence of bio-11-dUTP and hybridized to human metaphase cells prepared from phytohemagglutinin-stimulated peripheral blood lymphocytes as described previously. Hybridization of the OPRK1-specific probe [lambda]hSR4-1 DNA to normal human metaphase chromosomes resulted in specific labeling only of chromosome 8. Specific labeling of 8q11 was observed on all 4 (6 cells), 3 (9 cells), 2 (9 cells), or 1 (1 cell) chromatid of the chromosome 8 homologs in 25 cells examined. Of 72 signals observed, 70 were located at 8q11. 1 signal was located at 7q11 and at 12p11. In most cells, the signal on 8q was located at 8q11.2. 7 refs., 1 fig.
- OSTI ID:
- 6443558
- Journal Information:
- Genomics; (United States), Journal Name: Genomics; (United States) Vol. 19:3; ISSN GNMCEP; ISSN 0888-7543
- Country of Publication:
- United States
- Language:
- English
Similar Records
Localization of the genes encoding two transcription factors, LMX1 and CDX3, regulating insulin gene expression to human chromosomes 1 and 13
Localization of a candidate colon tumor-suppressor gene (DRA) to 7q22-q31. 1 by fluorescence in situ hybridization