Detecting marker-QTL linkage and estimating QTL gene effect and map location using a saturated genetic map

Journal Article · Thu Jul 01 04:00:00 EDT 1993 · Genetics; (United States)

OSTI ID:6441422

Darvasi, A; Weinreb, A; Minke, V; Soller, M ^[1]; Weller, J I ^[2]

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1. Hebrew Univ. of Jerusalem (Israel)
2. Animal Science Institute, Bet Dagan (Israel)

A simulation study was carried out on a backcross population in order to determine the effect of marker spacing, gene effect and population size on the power of marker-quantitative trait loci (QTL) linkage experiments and on the standard error of maximum likelihood estimates (MLE) of QTL gene effect and map location. Power of detecting a QTL was virtually the same for a marker spacing of 10 cM as for an infinite number of markers and was only slightly decreased for marker spacing of 20 or even 50 cM. The advantage of using interval mapping as compared to single-marker analysis was slight. [open quotes]Resolving power[close quotes] of a marker-QTL linkage experiment was defined as the 95% confidence interval for the QTL map location that would be obtained when scoring an infinite number of markers. It was found that reducing marker spacing below the resolving power did not add appreciably to narrowing the confidence interval. Thus, the 95% confidence interval with infinite markers sets the useful marker spacing for estimating QTL map location for a given population size and estimated gene effect. 27 refs., 2 figs., 4 tabs.

• OSTI ID: 6441422
• Journal Information: Genetics; (United States), Journal Name: Genetics; (United States) Vol. 134:3; ISSN GENTAE; ISSN 0016-6731
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL INDICATORS
COMPUTERIZED SIMULATION
DISTRIBUTION
GENETIC MAPPING
MAPPING
MATHEMATICAL MODELS
POPULATIONS
SIMULATION
SIZE
SPATIAL DISTRIBUTION
STATISTICAL MODELS