Cytokine production in BALB/c mice immunized with radiation attenuated third stage larvae of the filarial nematode, Brugia pahangi

Journal Article · Mon Feb 15 00:00:00 EST 1993 · Journal of Immunology; (United States)

OSTI ID:6441397

Bancroft, A J; Devaney, E ^[1]; Grencis, R K; Else, K J ^[2]

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1. Liverpool School of Tropical Medicine, Liverpool (United Kingdom)
2. Univ. of Manchester (United Kingdom)

BALB/c mice immunized with radiation-attenuated third stage larvae of the filarial nematode Brugia pahangi are strongly immune to challenge infection. Investigation of the profile of cytokines secreted by spleen cells from immune mice stimulated in vitro with either parasite Ag or with Con A revealed high levels of IL-5 and IL-9 and moderate levels of IL-4. In contrast, secretion of IFN-[gamma] by spleen cells from immune animals was negligible. Spleen cells from control mice secreted low levels of all cytokines assayed. Levels of parasite-specific IgE were significantly elevated in immune animals and a peripheral blood eosinophilia was observed, which exhibited a biphasic distribution. Our results are consistent with the preferential expansion of Th2 cells in immune animals and provide the basis for dissecting the means by which radiation-attenuated larvae of filarial nematodes stimulate immunity. 5l refs., 3 figs., 3 tabs.

• OSTI ID: 6441397
• Journal Information: Journal of Immunology; (United States), Vol. 150:4; ISSN 0022-1767
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
LARVAE
BIOLOGICAL RADIATION EFFECTS
LYMPHOKINES
SECRETION
MICE
IMMUNE REACTIONS
NEMATODES
IMMUNITY
IMMUNOGLOBULINS
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560152* - Radiation Effects on Animals- Animals
551000 - Physiological Systems