Neoplastic transformation dose response of oncogene-transfected rat embryo cells by gamma rays or 6 MeV alpha particles

Journal Article · Fri Apr 01 04:00:00 EST 1994 · Radiation Research; (United States)

DOI:https://doi.org/10.2307/3578849· OSTI ID:6440515

Ling, C C; Weiss, H; Strauss, A; Endlich, B; Sheh, Y; Wei, J X; Orazem, J ^[1]

---

1. Memorial Sloan-Kettering Cancer Center, New York, NY (United States)

We measured a dose-response relationship for induction of neoplastic transformation by 6 MeV [alpha] particles and [sup 137] Cs [gamma] rays in REC:myc and REC:ras cells, that is, rat embryo cells (REC) transfected with the c-myc or the Ha-ras oncogenes. The 6 MeV [alpha] particles simulated [sup 222]Rn emissions for risk assessment relative to low-LET radiations. The dose of [gamma] rays was approximately twice that of [alpha] particles for a neoplastic transformation frequency of 10[sup [minus]3]. The survival of the REC cells containing oncogenes was comparable to that of the commonly used C3H 10T1/2 cells for the same dose, but the former were more refractory to radiation-induced neoplastic transformation. Neoplastic transformation frequency measured in REC cells was 3 times lower than those typically measured in C3H 10T1/2 cells at a [gamma]-ray dose of 6 Gy, and 5-10 times lower at an [alpha]-particle dose of 3 Gy. 29 refs., 3 figs., 2 tabs.

• DOE Contract Number: FG02-89ER60903
• OSTI ID: 6440515
• Journal Information: Radiation Research; (United States), Journal Name: Radiation Research; (United States) Vol. 138:1; ISSN 0033-7587; ISSN RAREAE
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALPHA PARTICLES
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CELL TRANSFORMATIONS
CHARGED PARTICLES
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
EMBRYONIC CELLS
GAMMA RADIATION
GENES
IONIZING RADIATIONS
MUTANTS
ONCOGENES
ONCOGENIC TRANSFORMATIONS
RADIATION EFFECTS
RADIATION INDUCED MUTANTS
RADIATIONS