Studies of serotonin uptake and serotonin/sub 1A/ receptor using photoaffinity probes

Book · Wed Jan 01 00:00:00 EST 1986

OSTI ID:6439353

Lee, J D

The serotonergic system in the central nervous system (CNS) has been implicated in many physiological functions. The objectives of this study are: (1) to develop a new photoaffinity probe that can be used to identify the protein associated with the substrate binding site of the 5-HT uptake carrier, (2) to investigate the existence of different conformational states of the 5-HT carrier, (3) to solubilize the 5-HT/sub 1a/ receptor proteins from bovine hippocampus, and (4) to identify the 5-HT/sub 1a/ receptor proteins using a new photoaffinity probe, i.e., 1-(2-(4-aminophenyl)ethyl)4-(3-trifluoromethylphenyl)piperazine ((/sup 3/H)p-azido-PAPP), with high affinity for 5-HT/sub 1a/ receptor.

• OSTI ID: 6439353
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CENTRAL NERVOUS SYSTEM
BIOCHEMISTRY
SEROTONIN
RECEPTORS
UPTAKE
AFFINITY
BIOCHEMICAL REACTION KINETICS
BRAIN
CATTLE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
AMINES
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
AZAARENES
AZOLES
BODY
CHEMISTRY
DOMESTIC ANIMALS
DRUGS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INDOLES
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
NERVOUS SYSTEM
NEUROREGULATORS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PROTEINS
PYRROLES
RADIOPROTECTIVE SUBSTANCES
REACTION KINETICS
RUMINANTS
SYMPATHOMIMETICS
TRYPTAMINES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques