Enkephalin in the goldfish retina

Journal Article · Mon Dec 01 04:00:00 EST 1986 · Cell. Mol. Neurobiol.; (United States)

DOI:https://doi.org/10.1007/BF00711404· OSTI ID:6438638

Su, Y Y; Fry, K R; Lam, D M; Watt, C B

Enkephalin-like immunoreactive amacrine cells were visualized using the highly sensitive avidin-biotin method. The somas of these cells were situated in the inner nuclear and ganglion cell layers. Enkephalin-stained processes were observed in layers 1, 3, and 5 of the inner plexiform layer. The biosynthesis of sulfur-containing compounds in the goldfish retina was studied by means of a pulse-chase incubation with /sup 35/S-methionine. A /sup 35/S-labeled compound, which comigrated with authentic Met5-enkephalin on high-performance liquid chromatography (HPLC), was synthesized and was bound competitively by antibodies to enkephalin and by opiate receptors. This compound was tentatively identified as Met5-enkephalin. The newly synthesized /sup 35/S-Met5-enkephalin was released upon depolarization of the retina with a high K+ concentration. This K+-stimulated release was greatly suppressed by 5 mM Co/sup 2 +/, suggesting that the release was Ca/sup 2 +/ dependent. Using a double-label technique, enkephalin immunoreactivity and gamma-aminobutyric acid (GABA) uptake were colocalized to some amacrine cells, whereas others labeled only for enkephalin or GABA. The possible significance of enkephalin-GABA interactions is also discussed.

• Research Organization: Baylor College of Medicine, Houston, TX
• OSTI ID: 6438638
• Journal Information: Cell. Mol. Neurobiol.; (United States), Journal Name: Cell. Mol. Neurobiol.; (United States) Vol. 4; ISSN CMNED
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METAL COMPOUNDS
AMINO ACIDS
AMINOBUTYRIC ACID
ANIMALS
AQUATIC ORGANISMS
AUTONOMIC NERVOUS SYSTEM AGENTS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
BODY
BODY AREAS
CALCIUM COMPOUNDS
CARBOXYLIC ACIDS
CATIONS
CHARGED PARTICLES
CHEMISTRY
COBALT COMPOUNDS
CPB
DAYS LIVING RADIOISOTOPES
DOUBLE LABELLING
DRUGS
ENDORPHINS
ENKEPHALINS
EVEN-ODD NUCLEI
EYES
FACE
FISHES
GOLDFISH
HEAD
IMMUNE REACTIONS
IONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
LABELLING
LIGHT NUCLEI
LIPOTROPIC FACTORS
METHIONINE
NEUROREGULATORS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
PEPTIDES
POLYPEPTIDES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RETINA
SENSE ORGANS
SULFUR 35
SULFUR ISOTOPES
TRANSITION ELEMENT COMPOUNDS
TRITIUM COMPOUNDS
VERTEBRATES