Phosphatidylinositol cycle metabolites in Samanea saman Pulvini
The major metabolites of the phosphatidylinositol cycle from extracts of (/sup 32/PO/sub 4/)- and (/sup 3/H)-inositol-labeled Samanea saman pulvini were separated. The membrane localized phosphoinositides were separated by thin layer chromatography, identified by comparison with purified lipid standards, and quantitated based on incorporation of radiolabel. The ratio of radioactivity in phosphatidylinositol:phosphatidylinositol 4-phosphate:phosphatidylinositol 4,5-bisphosphate is about 32:8:1. The aqueous inositol phosphates were separated by anion exchange chromatography using conventional liquid chromatography and by high performance liquid chromatography (HPLC) and were identified by comparison with standards. Analysis by HPLC reveals that /sup 32/P-labeled pulvini have inositol 1-phosphate, inositol 1,4-bisphosphate, and inositol 1,4,5-trisphosphate that co-migrate with red blood cell inositol phosphates, but /sup 3/H-inositol-labeled pulvini appear to have a variant profile.
- Research Organization:
- Univ. of Connecticut, Storrs
- OSTI ID:
- 6438370
- Journal Information:
- Plant Physiol.; (United States), Journal Name: Plant Physiol.; (United States) Vol. 83:3; ISSN PLPHA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
ESTERS
FRACTIONATION
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LEGUMINOSAE
LIGHT NUCLEI
LIPIDS
LIQUID COLUMN CHROMATOGRAPHY
METABOLISM
METABOLITES
MICROORGANISMS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
OXYGEN COMPOUNDS
PHOSPHATES
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
PLANTS
RADIOISOTOPES
RHIZOBIUM
SEPARATION PROCESSES
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
TRITIUM COMPOUNDS