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Immunochemistry of sea anemone toxins: structure-antigenicity relationships and toxin-receptor interactions probed by antibodies specific for one antigenic

Journal Article · · Biochemistry; (United States)
OSTI ID:6437898
; ; ; ;

Two antibody subpopulations directed against Anemonia sulcata toxin I or II have been purified by immunoaffinity chromatography. These antibodies are specific for a single antigenic region and were used in a structure-antigenicity relationship study using homologous toxins and chemically modified derivatives of A. sulcata toxin II. Asp-7 and/or Asp=9 and Gln-47 of toxin II were found to be implicated in the antigenic region recognized by the two antibody subpopulations. On the contrary, Arg-14, Lys-35, -36, and -46, and ..cap alpha..-NH/sub 2/ of the glycine residue of A. sulcata toxin II are not involved in the corresponding antigenic region. When assayed for interaction with the sodium channel, the antigenic region of toxin II, including Asp-9 and Gln-47, appeared fully accessible to its specific antibodies, suggesting that it is not involved in the binding of the toxin to its receptor.

Research Organization:
INSERM U 172, Marseille, France
OSTI ID:
6437898
Journal Information:
Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 25:22; ISSN BICHA
Country of Publication:
United States
Language:
English

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59 BASIC BIOLOGICAL SCIENCES
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ANTIGEN-ANTIBODY REACTIONS
ANTIGENS
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IODINE ISOTOPES
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NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PHOSPHORS
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RADIOASSAY
RADIOIMMUNOASSAY
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STRUCTURE-ACTIVITY RELATIONSHIPS
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
VALENCE