Germ line transmission of a yeast artificial chromosome spanning the murine [alpha][sub 1](I) collagen locus

Journal Article · Fri Mar 26 00:00:00 EST 1993 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.8096090· OSTI ID:6434257

Strauss, W M; Dausman, J; Beard, C; Jaenisch, R ^[1]; Johnson, C; Lawrence, J B ^[2]

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1. Massachusetts Inst. of Technology, Cambridge (United States)
2. Univ. of Massachusetts Medical School, Worcester (United States)

Molecular complementation of mutant phenotypes by transgenic technology is a potentially important tool for gene identification. A technology was developed to allow the transfer of a physically intact yeast artificial chromosome (YAC) into the germ line of the mouse. A purified 150-kilobase YAC encompassing the murine gene Col1a1 was efficiently introduced into embryonic stem (ES) cells via lipofection. Chimeric founder mice were derived from two transfected ES cell clones. These chimeras transmitted the full length transgene through the germ line, generating two transgenic mouse strains. Transgene expression was visualized as nascent transcripts in interphase nuclei and quantitated by ribonuclease protection analysis. Both assays indicated that the transgene was expressed at levels comparable to the endogenous collagen gene. 32 refs., 3 figs., 1 tab.

• OSTI ID: 6434257
• Journal Information: Science (Washington, D.C.); (United States), Vol. 259:5103; ISSN 0036-8075
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GENES
DETECTION
TRANSMISSION
MUTATIONS
TRANSGENIC MICE
CONSTRUCTION
CHIMERAS
CHROMOSOMES
COLLAGEN
DNA-CLONING
MICE
TRANSCRIPTION
YEASTS
ANIMALS
CLONING
DNA HYBRIDIZATION
EUMYCOTA
FUNGI
HYBRIDIZATION
MAMMALS
MICROORGANISMS
MOSAICISM
ORGANIC COMPOUNDS
PLANTS
PROTEINS
RODENTS
SCLEROPROTEINS
TRANSGENIC ANIMALS
VERTEBRATES
550400* - Genetics