Differential fluorochromasia of human lymphocytes as measured by flow cytometry

Journal Article · Fri Dec 31 23:00:00 EST 1976 · J. Histochem. Cytochem.; (United States)

DOI:https://doi.org/10.1177/25.7.70458· OSTI ID:6434192

Wilder, M E; Cram, L S

Peripheral human lymphocytes reacted with fluorescein diacetate and analyzed by flow cytometry produced a bimodal fluorescence distribution that was shown to be attributable to the differential staining of T and B lymphocytes. Lymphocytes were fractionated into rosetting (T cell) and nonrosetting (B cell) populations. Both subfractions were reacted with fluorescein diacetate and analyzed by flow cytometry. The rosetting fraction was more fluorescent than the nonrosetting fraction, and the analysis of an appropriate mixture of the subfractionated populations produced a fluorescence distribution very similar to that obtained with unfractionated lymphocytes.

• Research Organization: Los Alamos Scientific Lab., NM
• OSTI ID: 6434192
• Journal Information: J. Histochem. Cytochem.; (United States), Journal Name: J. Histochem. Cytochem.; (United States) Vol. 25:7; ISSN JHCYA
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
AROMATICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CELL FLOW SYSTEMS
CONNECTIVE TISSUE CELLS
CYTOLOGICAL TECHNIQUES
DYES
FLUORESCEIN
FLUORESCENCE
HYDROXY ACIDS
HYDROXY COMPOUNDS
LEUKOCYTES
LUMINESCENCE
LYMPHOCYTES
ORGANIC ACIDS
ORGANIC COMPOUNDS
PHENOLS
POLYPHENOLS
SOMATIC CELLS