Ultrastructural changes in leaf cells of ponderosa pine and cotton exposed to ozone

Technical Report · Fri Sep 01 04:00:00 EDT 1978

OSTI ID:6432040

Furr, M

Damage and modifications of plant cell structure by moderate to high levels of ozone were studied in ponderosa pine (Pinus ponderosa Laws.) and cotton (Gossypium hirsuta L. cv 'Acala SJ-2'). Plants were exposed to 0.20 ppM of ozone for six hrs daily. Visible symptoms of ozone damage in the pine included development of tip necrosis, chlorotic mottle, and necrotic lesions or bands in both current and one year old needles. In cotton seedlings, chlorosis developed in the upper cell layers of the leaves and cotyledons. Leaf blade islets were affected prior to any noticeable damage to the vascular systems and early cotyledon abscission was common in fumigated cotton seedlings. Electron microscope observations revealed that ozone altered membranes in the cytoplasmic organelles. Degradation of the granal and thylakoid systems of chloroplastids paralleled the concentration and duration of ozone fumigation. As associated accumulation of lipoidal or osmiophilic spheres in the chloroplastid stroma accompanied the modification of the thylakoid membranes in cotton. Both granal membranes and chloroplastid morphology were altered in ozone damaged pine needles.

• Research Organization: Paine Coll., Augusta, GA (USA)
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 6432040
• Report Number(s): UCRL-13909
• Country of Publication: United States
• Language: English

Related Subjects

550300 -- Cytology
553000 -- Agriculture & Food Technology
560303* -- Chemicals Metabolism & Toxicology-- Plants-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL EFFECTS
BIOMASS
CELL CONSTITUENTS
CELL MEMBRANES
CHLOROPLASTS
CHRONIC EXPOSURE
CONIFERS
COTTON PLANTS
CYTOPLASM
DISEASES
DOSE-RESPONSE RELATIONSHIPS
ELECTRON MICROSCOPY
ENERGY SOURCES
LEAVES
MEMBRANES
MICROSCOPY
MORPHOLOGICAL CHANGES
NECROSIS
ORGANOIDS
OZONE
PATHOLOGICAL CHANGES
PINES
PLANT CELLS
PLANTS
RENEWABLE ENERGY SOURCES
TOXICITY
TREES
ULTRASTRUCTURAL CHANGES