Colocalization of barley lectin and sporamin in vacuoles of transgenic tobacco plants

Journal Article · Mon Feb 01 04:00:00 EST 1993 · Plant Physiology; (United States)

DOI:https://doi.org/10.1104/pp.101.2.451· OSTI ID:6430035

Schroeder, M R; Borkhsenious, O N; Raikhel, N V ^[1]; Matsuoka, K; Nakamura, K ^[2]

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1. Michigan State Univ., East Lansing (United States)
2. Nagoya Univ. (Japan)

Various targeting motifs have been identified for plant proteins delivered to the vacuole. For barley (Hordeum vulgare) lectin, a typical Gramineae lectin and defense-related protein, the vacuolar information is contained in a carboxyl-terminal propeptide. In contrast, the vacuolar targeting information of sporamin, a storage protein from the tuberous roots of the sweet potato (Ipomoea batatas), is encoded in an amino-terminal propeptide. Both proteins were expressed simultaneously in transgenic tobacco plants to enable analysis of their posttranslational processing and subcellular localization by pulse-chase labeling and electron-microscopic immunocytochemical methods. The pulse-chase experiments demonstrated that processing and delivery to the vacuole are not impaired by the simultaneous expression of barley lectin and sporamin. Both proteins were targeted quantitatively to the vacuole, indication that the carboxyl-terminal and amino-terminal propeptided are equally recognized by the vacuolar protein-sorting machinery. Double-labeling experiments showed that barley lectin and sporamin accumulate in the same vacuole of transgenic tobacco (Nicotiana tabacum) leaf and root cells. 35 refs., 5 figs., 3 tabs.

• OSTI ID: 6430035
• Journal Information: Plant Physiology; (United States), Journal Name: Plant Physiology; (United States) Vol. 101:2; ISSN 0032-0889; ISSN PLPHAY
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
BARLEY
BUILDUP
CELL CONSTITUENTS
CEREALS
GRAMINEAE
LILIOPSIDA
MAGNOLIOPHYTA
MAGNOLIOPSIDA
NICOTIANA
ORGANIC COMPOUNDS
PEPTIDES
PLANT CELLS
PLANTS
POLYPEPTIDES
PROTEINS
ROOTS
TOBACCO