Effect of host lex, recA, recF, and uvrD genotypes on the ultraviolet light-protecting and related properties of plasmid R46 in Escherichia coli
The ability of plasmid R46 to reduce the lethal but enhance the mutagenic effect of ultraviolet (uv) irradiation was tested in sets of Escherichia coli K-12 derivatives, wild type or with different mutations affecting DNA repair capacity, but otherwise isogenic. uv protection and enhancement of uv mutagenic effect were obtained in uvrA6, uvrB5, uvrD3, and recF143 hosts, but not in a recA56 strain. The plasmid gave some uv protection in two lexA1 and two lexA101 strains and in one lexA102 host, but produced no such effect in another lexA102 host. The plasmid restored uv mutagenic effect in a lexB30 strain, the yield of induced mutants per survivor of irradiation (10 J/m/sup 2/) being about the same for the lexB30(R46) and lex/sup +/(R46) strains; by contrast the plasmid, though it reduced the uv sensitivity of the lexB30 strain, did not make it as uv-resistant as the lex/sup +/R/sup -/ strain.
- Research Organization:
- Stanford Univ., CA
- OSTI ID:
- 6424923
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 137:2; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
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Ultraviolet light protection, enhancement of ultraviolet light mutagenesis, and mutator effect of plasmid R46 in Salmonella typhimurium. [X radiation]
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Related Subjects
560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
DATA
DATA FORMS
DNA
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
EXPERIMENTAL DATA
GENOTYPE
GRAPHS
INFORMATION
ISOLATED VALUES
MICROORGANISMS
MUTATIONS
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
PLASMIDS
RADIATION EFFECTS
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
ULTRAVIOLET RADIATION