Effect of humic fractions and clay on biodegradation of phenanthrene by a Pseudomonas fluorescens strain isolated from soil

Journal Article · Sat Aug 01 00:00:00 EDT 1998 · Applied and Environmental Microbiology

OSTI ID:642349

Ortega-Calvo, J J; Saiz-Jimenez, C ^[1]

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1. C.S.I.C., Seville (Spain). Inst. de Recursos Naturales y Agrobiologia

The mineralization of phenanthrene in pure cultures of a Pseudomonas fluorescens strain, isolated from soil, was measured in the presence of soil humic fractions and montmorillonite. Humic acid and clay, either separately or in combination, shortened the acclimation phase. A higher mineralization rate was measured in treatments with humic acid at 100 {micro}g/ml. Humic acid at 10 {micro}g/ml stimulated the transformation only in the presence of 10 g of clay per liter. The authors suggest that sorption of phenanthrene to these soil components may result in a higher concentration of substrate in the vicinity of the bacterial cells and therefore may increase its bioavailability.

• OSTI ID: 642349
• Journal Information: Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 8 Vol. 64; ISSN AEMIDF; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
BIODEGRADATION
HUMIC ACIDS
PHENANTHRENE
PSEUDOMONAS
REMEDIAL ACTION
SOILS