Oxidation of trichloroethylene, 1,1-dichloroethylene, and chloroform by toluene/o-xylene monooxygenase from Pseudomonas stutzeri OX1

Journal Article · Sat Aug 01 04:00:00 EDT 1998 · Applied and Environmental Microbiology

OSTI ID:642346

Chauhan, S; Wood, T K ^[1]; Barbieri, P ^[2]

---

1. Univ. of California, Irvine, CA (United States). Dept. of Chemical and Biochemical Engineering
2. Univ. degli Studi di Milano, Milan (Italy). Dept. di Genetica e di Biologia dei Microrganismi

Toluene/o-xylene monooxygenase (ToMO) from Pseudomonas stutzeri OX1, which oxidizes toluene and o-xylene, was examined for its ability to degrade the environmental pollutants trichloroethylene (TCE), 1,1-dichloroethylene (1,1-DCE), cis-1,2-DCE, trans-1,2-DCE, chloroform, dichloromethane, phenol, 2,4-dichlorophenol, 2,4,5-trichlorophenol, 2,4,6-trichlorophenol, 2,3,5,6-tetrachlorophenol, and 2,3,4,5,6-pentachlorophenol. Escherichia coli JM109 that expressed ToMO from genes on plasmid pBZ1260 under control of the lac promoter degraded TCE, 1,1-DCE, and chloroform at initial rates of 3.1, 3.6, and 1.6 nmol, respectively. Stoichiometric amounts of chloride release were seen, indicating mineralization. Thus, the substrate range of ToMO is extended to include aliphatic chlorinated compounds.

• OSTI ID: 642346
• Journal Information: Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 8 Vol. 64; ISSN AEMIDF; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
BIODEGRADATION
CHLORINATED ALIPHATIC HYDROCARBONS
CHLOROFORM
ESCHERICHIA COLI
GENE REGULATION
OXYGENASES
PSEUDOMONAS
REMEDIAL ACTION