A novel tumor necrosis factor-responsive transcription factor which recognizes a regulatory element in hemopoietic growth factor genes

Journal Article · Fri Jun 01 04:00:00 EDT 1990 · Molecular and Cellular Biology; (USA)

DOI:https://doi.org/10.1128/MCB.10.6.2950· OSTI ID:6422532

Shannon, M F; Pell, L M; Kuczek, E S; Occhiodoro, F S; Dunn, S M; Vadas, M A ^[1]; Lenardo, M J ^[2]

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1. Div. of Human Immunology, Institute of Medical and Veterinary Science, Frame Road, Adelaide 5001 (AU)
2. Lab. of Immunology, National Institute of Allergy and Infectious Diseases, Bethesda, MD (US)

A conserved DNA sequence element, termed cytokine 1 (CK-1), is found in the promoter regions of many hemopoietic growth factor (HGF) genes. Mutational analyses and modification interference experiments show that this sequence specifically binds a nuclear transcription factor, NF-GMa, which is a protein with a molecular mass of 43 kilodaltons. It interacts with different affinities with the CK-1-like sequence from a number of HGF genes, including granulocyte macrophage colony-stimulating factor (GM-CSF), granulocyte (G)-CSF, interleukin 3 (IL-3), and IL-5. The authors show that the level of NF-GMa binding is induced in embryonic fibroblasts by tumor necrosis factor {alpha} (TNF-{alpha}) treatment and that the CK-1 sequence from the G-CSF gene is a TNF-{alpha}-responsive enhancer in these cells.

• OSTI ID: 6422532
• Journal Information: Molecular and Cellular Biology; (USA), Journal Name: Molecular and Cellular Biology; (USA) Vol. 10:6; ISSN MCEBD; ISSN 0270-7306
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
ANIMAL CELLS
ANTIGEN-ANTIBODY REACTIONS
BODY
DATA
DNA SEQUENCING
EXPERIMENTAL DATA
GENE REGULATION
GENE REPRESSORS
GENES
GROWTH FACTORS
HEMATOPOIETIC SYSTEM
INFORMATION
MITOGENS
NECROSIS
NUCLEOPROTEINS
NUMERICAL DATA
ORGANIC COMPOUNDS
PATHOLOGICAL CHANGES
PROTEINS
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION FACTORS
TUMOR CELLS