Mutagenic effect of radionuclides incorporated into DNA of Drosophila melanogaster. Progress report, 1977--1978. [Effects of tritium decay in labelled sperm]

Technical Report · Sun Jan 01 04:00:00 EST 1978

DOI:https://doi.org/10.2172/6419745· OSTI ID:6419745

Lee, W. R.

Three experiments were conducted in which Drosophila melanogaster males were labeled with (5-/sup 3/H)-deoxycytidine, (8-/sup 3/H)-deoxyguanosine, or L-(3-/sup 3/H)-arginine in the larval stage. In the first two experiments with 5-/sup 3/H-dC and 8-/sup 3/H-dG, the labeled males upon eclosion were divided into two groups: 1) those mated to females that from emergence as adults were sustained on a media deficient in protein, called sugar agar media; and 2) those mated to females that were on a standard nutritious media. All males from the arginine experiment were mated to females stored on the standard media. The labeled males were allowed to mate for two days during which time the females stored on standard media deposited fertile eggs. Those F/sub 1/ progenies developing from the brood of eggs laid during the first 48 hrs following mating were called the non-stored group, as the spermatozoa were not stored in the female for more than 48 hrs. Progenies of males that were mated to females sustained on the sugar agar media were referred to as the stored group because the females on the sugar agar did not lay eggs. After 14 days the females were transferred to regular corn meal agar to induce egg laying. The average time of storage of sperm in the females' seminal receptacles for the stored group was 18 days. The progenies from the non-stored and stored broods were analyzed for mutations thus giving a comparison of the mutation frequency for each class of mutants detected before and after storage of the tritium-labeled sperm. The difference between the mutation frequency following storage and that before storage is the increase in mutations attributed to tritium disintegrations occurring during the storage period. One determination for each experiment, 5-/sup 3/H-dC, 8-/sup 3/H-dG, or /sup 3/H-Arg. has been made.

• Research Organization: Louisiana State Univ., Baton Rouge (USA). Dept. of Zoology and Physiology
• OSTI ID: 6419745
• Report Number(s): ORO-3728-11
• Country of Publication: United States
• Language: English

Related Subjects

560162* -- Radionuclide Effects
Kinetics
& Toxicology-- Animals
Plants
Microorganisms
& Cells
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMALS
ARTHROPODS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
DECAY
DROSOPHILA
FLIES
FRUIT FLIES
GAMETES
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
GERM CELLS
HYDROGEN ISOTOPES
INGESTION
INSECTS
INTAKE
INTERNAL IRRADIATION
INVERTEBRATES
IRRADIATION
ISOTOPES
LABELLED COMPOUNDS
LABELLING
LIGHT NUCLEI
MALES
MUTAGENESIS
MUTATION FREQUENCY
MUTATIONS
NUCLEI
ODD-EVEN NUCLEI
PROGENY
RADIATION EFFECTS
RADIOINDUCTION
RADIOISOTOPES
SPERMATOZOA
TRITIUM
TRITIUM COMPOUNDS
YEARS LIVING RADIOISOTOPES