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X-ray-induced breakage and rejoining of human interphase chromosomes

Journal Article · · Science (Washington, D.C.); (United States)
;
A method was developed for the high-resolution measurement of breaks in prematurely condensed chromosomes at the G/sub 1/ phase of the cell cycle. The dose response for fragments (breaks) produced immediately after x-irradiation of confluent cultures of normal human cells was linear down to 10.9 rad (0.109 Gy) and extrapolated to zero effect at zero dose. The curve had a slope of 0.063 breaks per cell per rad, which is at least an order of magnitude greater than that for breaks scored in the same cells after they have progressed to mitosis following subculture. When incubated at 37/sup 0/C half of the breaks disappeared in 2 hours. A slower, perhaps nonrejoining component was apparent at later incubation times. The initial rate of break rejoining was similar to the rate of increase in survival after incubation because of the repair of potentially lethal damage and is also in close agreement with recently reported values for the rejoining of double-strand breakage in DNA.
Research Organization:
Colorado State Univ., Fort Collins
OSTI ID:
6411975
Journal Information:
Science (Washington, D.C.); (United States), Journal Name: Science (Washington, D.C.); (United States) Vol. 222; ISSN SCIEA
Country of Publication:
United States
Language:
English

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Related Subjects

560111* -- Radiation Effects on Biochemicals-- In Vitro-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CYCLE
CELL DIVISION
CHROMOSOMAL ABERRATIONS
CHROMOSOME BREAKAGE
CHROMOSOMES
DNA
DNA REPAIR
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
INCUBATION
IONIZING RADIATIONS
MAMMALS
MAN
MEASURING METHODS
MITOSIS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PRIMATES
RADIATIONS
RADIOINDUCTION
RADIOSENSITIVITY
RECOVERY
REPAIR
SURVIVAL CURVES
TEMPERATURE EFFECTS
VERTEBRATES
X RADIATION