Characterization of (/sup 125/I)omega-conotoxin binding to brain N calcium channels and (-)(/sup 3/H) desmethoxyverapamil binding to novel calcium channels in osteoblast-like osteosarcoma cells
Thesis/Dissertation
·
OSTI ID:6411821
This dissertation provides molecular evidence for a diversity of Ca/sup 2 +/ channels in neuronal and non-neuronal tissues. First, I demonstrated specific, reversible, saturable binding sites for omega (/sup 125/I)conotoxin GVIA (omega(/sup 125/I)CTX) in rat brain and rabbit sympathetic ganglion. Omega (/sup 125/I)CTX binding has a unique pharmacology, ion selectivity, and anatomical distribution in rat brain. Omega (/sup 125/I)CTX binding was solubilized, retaining an appropriate pharmacology and ion selectivity. Omega(/sup 125/I)CTX binding may be associated with a Ca/sup 2 +/ channel because the K/sub D/ of omega (/sup 125/I)CTX is similar to the IC/sub 50/ of inhibition of depolarization-induced /sup 45/Ca/sup 2 +/ flux into rat brain synaptosomes. Specific (-)(/sup 3/H)desmethoxyverapamil ((-)(/sup 3/H)DMV) binding sites were demonstrated on osteoblast-like osteosarcoma cell membranes.
- Research Organization:
- Johns Hopkins Univ., Baltimore, MD (USA)
- OSTI ID:
- 6411821
- Country of Publication:
- United States
- Language:
- English
Similar Records
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Journal Article
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OSTI ID:6696124
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· Biochemistry; (United States)
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·
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· Proceedings of the National Academy of Sciences of the United States of America; (United States)
·
OSTI ID:5823159
Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CALCIUM 45
CALCIUM COMPOUNDS
CALCIUM ISOTOPES
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MEMBRANE TRANSPORT
MEMBRANES
NERVE CELLS
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RABBITS
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SOMATIC CELLS
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS
VASODILATORS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH ISOTOPES
ALKALINE EARTH METAL COMPOUNDS
ANIMAL CELLS
ANIMALS
ANTIGENS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CALCIUM 45
CALCIUM COMPOUNDS
CALCIUM ISOTOPES
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CELL MEMBRANES
CENTRAL NERVOUS SYSTEM
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
IODINE 125
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
MEMBRANE PROTEINS
MEMBRANE TRANSPORT
MEMBRANES
NERVE CELLS
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RABBITS
RADIOISOTOPES
RATS
REACTION KINETICS
RECEPTORS
RODENTS
SOMATIC CELLS
TOXIC MATERIALS
TOXINS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS
VASODILATORS
VERTEBRATES