sup 32 P-Postlabeling measurement of adenine N-1-oxide in cellular DNA exposed to hydrogen peroxide
Journal Article
·
· Chemical Research in Toxicology; (USA)
OSTI ID:6411490
- Centre d'Etudes Nucleaires de Grenoble (France)
A {sup 32}P-postlabeling assay has been developed for monitoring the formation within DNA of adenine N-1-oxide, the specific H{sub 2}O{sub 2}-mediated oxidation product under nonradical conditions. This has required the chemical synthesis of both 2{prime}-deoxyadenosine N-1-oxide 3{prime}-monophosphate and 2{prime}-deoxyadenosine N-1-oxide 5{prime}-monophosphate, the substrate and the product of polynucleotide kinase mediated phosphorylation. Isolation of the substrate from the other nucleotides was found to be necessary in order to improve the rate of phosphorylation and to prevent self-radiolysis processes. ({sup 32}P)-2{prime}-deoxyadenosine N-1-oxide 5{prime}-monophosphate was obtained after successive ion exchange and reverse-phase HPLC and was characterized by a microreaction. The sensitivity of the assay, which is close to 1 modified adenine N-1-oxide/10{sup 6} bases, allowed the determination of this lesion within the DNA of cells exposed to nonlethal levels of H{sub 2}O{sub 2}.
- OSTI ID:
- 6411490
- Journal Information:
- Chemical Research in Toxicology; (USA), Journal Name: Chemical Research in Toxicology; (USA) Vol. 3:2; ISSN CRTOE; ISSN 0893-228X
- Country of Publication:
- United States
- Language:
- English
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Mon Jan 28 23:00:00 EST 1991
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·
OSTI ID:5510474
/sup 32/P-Postlabeling test for covalent DNA binding of chemicals in vivo: Application to a variety of aromatic carcinogens and methylating agents
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OSTI ID:6850981
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·
OSTI ID:5068033
Related Subjects
560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADENINES
AMINES
ANTIMETABOLITES
AROMATICS
AZAARENES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL REACTIONS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DNA
DRUGS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
ION EXCHANGE
ISOTOPES
LIGHT NUCLEI
LIQUID COLUMN CHROMATOGRAPHY
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDATION
OXYGEN COMPOUNDS
PEROXIDES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PURINES
RADIOISOTOPES
SEPARATION PROCESSES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADENINES
AMINES
ANTIMETABOLITES
AROMATICS
AZAARENES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CHEMICAL REACTIONS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DNA
DRUGS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROGEN PEROXIDE
ION EXCHANGE
ISOTOPES
LIGHT NUCLEI
LIQUID COLUMN CHROMATOGRAPHY
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXIDATION
OXYGEN COMPOUNDS
PEROXIDES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PURINES
RADIOISOTOPES
SEPARATION PROCESSES