Effect of ultrasound on the separation of DNA fragments in agarose gel electrophoresis

Journal Article · Fri Jun 01 00:00:00 EDT 1990 · Analytical Chemistry (Washington); (USA)

DOI:https://doi.org/10.1021/ac00210a019· OSTI ID:6410246

Ma, Yinfa; Yeung, E S ^[1]

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1. Iowa State Univ., Ames (USA)

Since its first use in 1966 interest in and the applications of electrophoresis of DNA fragments in agarose gel have grown rapidly. Nowadays, agarose gel electrophoresis has become a standard technique with high resolving power for the analysis of DNA structure, for example for the determination of the length of DNA fragments obtained by the action of restriction enzymes. The electrophoretic mobility ({mu}) of DNA fragments is influenced by various parameters-molecular weight, gel concentration, temperature, electric field, and DNA-agarose affinity. A comprehensive study of the influence of these main parameters has been reported. In this paper, the authors investigate a new effect on the electrophoretic mobility of DNA fragments in agarose gels, viz. the influence of ultrasound.

• OSTI ID: 6410246
• Journal Information: Analytical Chemistry (Washington); (USA), Vol. 62:11; ISSN 0003-2700
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
DNA
ELECTROPHORESIS
CALCULATION METHODS
DNA SEQUENCING
EXPERIMENTAL DATA
GELS
SEPARATION PROCESSES
COLLOIDS
DATA
DISPERSIONS
INFORMATION
NUCLEIC ACIDS
NUMERICAL DATA
ORGANIC COMPOUNDS
STRUCTURAL CHEMICAL ANALYSIS
400102* - Chemical & Spectral Procedures
550200 - Biochemistry