Cell kinetic studies of the cytostatic and cytocidal effect of 1-beta-D-arabinofuranosylcytosine on the L1210 ascites tumor

Journal Article · Thu Mar 01 04:00:00 EST 1984 · Cancer Res.; (United States)

OSTI ID:6407397

Fietkau, R; Friede, H; Maurer-Schultze, B

The effect of 1-beta-D-arabinofuranosylcytosine (ara-C) on mouse L1210 ascites tumor cells was studied in vivo with cell kinetic methods. Injection of ara-C (200 mg/kg) leads to an immediate inhibition of DNA synthesis and, thus, to a block of G1 cells at the G1-S border. This dose of ara-C kills a considerable fraction (at least 54%) of the L1210 cells. A double-labeling experiment with (/sup 3/H)thymidine and (/sup 14/C)thymidine which permits the distinction of cells in different cycle phases shows that ara-C kills the majority of cells in S phase, while only a minor fraction of G1 cells are lethally damaged. A multicompartment model is utilized to derive from the results of the double-labeling experiment the rates of cell loss out of the different cycle phases. Eighty-seven percent of the cells that are in S phase at the time of ara-C application and 21% of the cells in G1 are killed by ara-C. The repopulation of the tumor starts from the remaining G1 cells. A mean life span of the necrotic cells of 1.9 hr is derived from the model.

• Research Organization: Universitaet Wuerzburg, Federal Republic of Germany
• OSTI ID: 6407397
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 44:3; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

550501 -- Metabolism-- Tracer Techniques
560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANTINEOPLASTIC DRUGS
ASCITES
AZINES
BIOLOGICAL EFFECTS
BIOSYNTHESIS
CARBON 14 COMPOUNDS
CELL KILLING
DNA
DRUGS
HETEROCYCLIC COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PATHOLOGICAL CHANGES
PYRIMIDINES
RIBOSIDES
SYMPTOMS
SYNTHESIS
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
TUMOR CELLS