Isolation of sugarcane bagasse hydrolyzate-tolerant yeast mutants by continuous selection
Hemicellulose, one of the major constituents of plant cell-wall materials, comprises up to 40% of agricultural residues and hardwoods. Upon hydrolysis, hemicellulose yields a mixture of carbohydrates of which D-xylose is the major component. Hemicellulose-derived carbohydrates can easily be obtained by use of dilute acids under mild hydrolysis conditions. These sugars as well as cellulose-derived carbohydrates, are potential substrates for ethanol production. Often during acid hydrolysis many potentially toxic chemicals are formed which have been found to inhibit yeast growth and ethanol production. It is, therefore, necessary to overcome the inhibitory effect before a fermentation can be implemented. In addition to these fermentation inhibitors, salts formed as a result of neutralization of acid hydrolyzate can also affect the yeasts, thereby decreasing the fermentation rate. Previously, we have shown that ethanol can be produced from sugarcane bagasse hemicellulose hydrolyzate by a xylose-fermentating yeast, Candida species XF217, after the hydrolyzate had been treated with ion-exchange resins. This communication describes the isolation of hydrolyzate-tolerant yeast strains by a continuous adaptation and selection technique and also the growth and fermentative abilities of the strain, P11-20 in neutralized hydrolyzate.
- Research Organization:
- Purdue Univ., West Lafayette, IN
- OSTI ID:
- 6407265
- Report Number(s):
- CONF-840509-
- Journal Information:
- Biotechnol. Bioeng. Symp.; (United States), Vol. 16:14; Conference: 6. symposium on biotechnology for fuels and chemicals, Gatlinburg, TN, USA, 15 May 1984
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BAGASSE
ACID HYDROLYSIS
HEMICELLULOSE
YEASTS
BIOLOGICAL ADAPTATION
CELL WALL
CELLULOSE
ETHANOL
INHIBITION
PRODUCTION
SUBSTRATES
TOLERANCE
XYLOSE
AGRICULTURAL WASTES
ALCOHOLS
ALDEHYDES
CARBOHYDRATES
CELL CONSTITUENTS
CHEMICAL REACTIONS
DECOMPOSITION
FUNGI
HYDROLYSIS
HYDROXY COMPOUNDS
LYSIS
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC WASTES
PENTOSES
PLANTS
POLYSACCHARIDES
SACCHARIDES
SOLVOLYSIS
WASTES
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