Isolation of sugarcane bagasse hydrolyzate-tolerant yeast mutants by continuous selection

Conference · Sun Jan 01 04:00:00 EST 1984 · Biotechnol. Bioeng. Symp.; (United States)

OSTI ID:6407265

Lodics, T A; Gong, C S

Hemicellulose, one of the major constituents of plant cell-wall materials, comprises up to 40% of agricultural residues and hardwoods. Upon hydrolysis, hemicellulose yields a mixture of carbohydrates of which D-xylose is the major component. Hemicellulose-derived carbohydrates can easily be obtained by use of dilute acids under mild hydrolysis conditions. These sugars as well as cellulose-derived carbohydrates, are potential substrates for ethanol production. Often during acid hydrolysis many potentially toxic chemicals are formed which have been found to inhibit yeast growth and ethanol production. It is, therefore, necessary to overcome the inhibitory effect before a fermentation can be implemented. In addition to these fermentation inhibitors, salts formed as a result of neutralization of acid hydrolyzate can also affect the yeasts, thereby decreasing the fermentation rate. Previously, we have shown that ethanol can be produced from sugarcane bagasse hemicellulose hydrolyzate by a xylose-fermentating yeast, Candida species XF217, after the hydrolyzate had been treated with ion-exchange resins. This communication describes the isolation of hydrolyzate-tolerant yeast strains by a continuous adaptation and selection technique and also the growth and fermentative abilities of the strain, P11-20 in neutralized hydrolyzate.

• Research Organization: Purdue Univ., West Lafayette, IN
• OSTI ID: 6407265
• Report Number(s): CONF-840509-
• Conference Information: Journal Name: Biotechnol. Bioeng. Symp.; (United States) Journal Volume: 16:14
• Country of Publication: United States
• Language: English

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ACID HYDROLYSIS
AGRICULTURAL WASTES
ALCOHOLS
ALDEHYDES
BAGASSE
BIOLOGICAL ADAPTATION
CARBOHYDRATES
CELL CONSTITUENTS
CELL WALL
CELLULOSE
CHEMICAL REACTIONS
DECOMPOSITION
ETHANOL
FUNGI
HEMICELLULOSE
HYDROLYSIS
HYDROXY COMPOUNDS
INHIBITION
LYSIS
MICROORGANISMS
MONOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC WASTES
PENTOSES
PLANTS
POLYSACCHARIDES
PRODUCTION
SACCHARIDES
SOLVOLYSIS
SUBSTRATES
TOLERANCE
WASTES
XYLOSE
YEASTS