Genetic studies on plasmid-linked cadmium resistance in Staphylococcus aureus

Journal Article · Fri Dec 31 23:00:00 EST 1971 · J. Bacteriol.; (United States)

OSTI ID:6407237

Smith, K; Novick, R P

The genetic basis of cadmium resistance conferred by three penicillinase plasmids, PI/sub 524/, PI/sub 258/, and PII/sub 147/, of Staphylococcus aureus was examined by mutation, recombination, and deletion analysis. Three separate loci were identified: cadA, responsible for high-level resistance; cadB, giving a low-level resistance, nonadditive to cadA; and mad, a locus marginally decreasing the cadmium resistance of plasmid-positive staphylococci. The loci cadA and mad were present on all three plasmids, but cadB was only found on PII/sub 147/. Spontaneous deletions of mad involved up to three-fourths of the plasmic genome, which allowed derivation of a partial deletion map of PII/sub 147/, a plasmid with a contour length of 10.9 ..mu..m, corresponding to a molecular weight of 20.4 X 10/sup 6/.

• Research Organization: City of New York, Inc., NY
• OSTI ID: 6407237
• Journal Information: J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 112; ISSN JOBAA
• Country of Publication: United States
• Language: English

Related Subjects

550400 -- Genetics
560302* -- Chemicals Metabolism & Toxicology-- Microorganisms-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGY
CADMIUM
CELL CONSTITUENTS
CHROMOSOMAL ABERRATIONS
ELEMENTS
GENE RECOMBINATION
GENETIC EFFECTS
GENETIC MAPPING
GENETICS
MAPPING
METALS
MICROORGANISMS
MUTATIONS
PLASMIDS
STAPHYLOCOCCUS