Molecular cloning of a recA-like gene from the cyanobacterium Anabaena variabilis
Journal Article
·
· J. Bacteriol.; (United States)
A recA-like gene isolated from the cyanobacterium Anabaena variabilis was cloned and partially characterized. When introduced into Escherichia coli recA mutants, the 7.5-kilobase-pair plasmid-borne DNA insert restored resistance to methyl methanesulfonate and UV irradiation, as well as recombination proficiency when measured by Hfr-mediated conjugation. The cyanobacterial recA gene restored spontaneous but not mitomycin C-induced prophage production. Restriction analysis and subcloning yielded a 1.5-kilobase-pair Sau3A fragment which also restored methylmethane sulfonate resistance and coded for a 38- to 40-kilodalton polypeptide when expressed in an in vitro transcription-translation system.
- Research Organization:
- Univ. of Toronto, Ontario
- OSTI ID:
- 6405338
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 5; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550401 -- Genetics-- Tracer Techniques
560130* -- Radiation Effects on Microorganisms
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CLONING
CYANOBACTERIA
DNA REPAIR
ELECTROMAGNETIC RADIATION
ESTERS
GENE RECOMBINATION
GENES
METHYL METHANESULFONATE
MICROORGANISMS
MUTAGENS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
SULFONIC ACID ESTERS
TRANSCRIPTION
ULTRAVIOLET RADIATION
560130* -- Radiation Effects on Microorganisms
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CLONING
CYANOBACTERIA
DNA REPAIR
ELECTROMAGNETIC RADIATION
ESTERS
GENE RECOMBINATION
GENES
METHYL METHANESULFONATE
MICROORGANISMS
MUTAGENS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIATIONS
RADIOSENSITIVITY
RECOVERY
REPAIR
SULFONIC ACID ESTERS
TRANSCRIPTION
ULTRAVIOLET RADIATION