Picosecond transient absorption study of photodissociated carboxy hemoglobin and myoglobin
The optical transient absorption spectra at 30 ps and 6.5 ns after photolysis are compared for both carboxy hemoglobin (HbCO) and carboxy myoglobin (MbCO). Both 355- and 532-nm excitation pulses were used. In all cases the shapes of the optical difference spectra thus generated are stationary over the complete time-scale studied. The photolysis spectra for MbCO are not significantly different from the equilibrium difference spectra generated on the same picosecond spectrometer when measured to an accuracy of +/- 0.5 nm. In addition, spectral parameters for delegated HbCO generated on the same spectrometer but detected by two different techniques, either by a Vidicon detector or point by point with photomultiplier tubes, are reported; the results are different from some of the previously reported picosecond experiments.
- Research Organization:
- Univ. of Pennsylvania, Philadelphia (USA)
- OSTI ID:
- 6403518
- Journal Information:
- Biophys. J.; (United States), Journal Name: Biophys. J.; (United States) Vol. 54:3; ISSN BIOJA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ABSORPTION SPECTRA
ACCURACY
CARBOXYHEMOGLOBIN
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
DECOMPOSITION
GLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
KINETICS
MEASURING INSTRUMENTS
MYOGLOBIN
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHOTOLYSIS
PIGMENTS
PORPHYRINS
PROTEINS
SPECTRA
SPECTROMETERS
ULTRAVIOLET SPECTRA
ULTRAVIOLET SPECTROMETERS