Comparison of in vivo effects of human recombinant IL 1 and human recombinant IL 6 in mice
IL 1 and IL 6 share a number of biological activities, including induction of fever, neutrophilia and acute phase response, and IL 1 induces IL 6 production by fibroblasts and macrophages. Therefore, it was proposed that IL 6 mediates many of the activities of IL 1. To test this hypothesis in vivo, we assessed induction of IL 6 following IL 1 alpha administration to mice and tested IL 6 for radioprotection and induction of early (CSF) and late (fibrinogen and SAA) acute phase reactants. IL 1 alpha given to mice ip induced, in a dose dependent manner, detectable IL 6 in circulation, with maximal titers at 2-4 hrs. However, unlike IL 1 which is 10-1000 ng/mouse of human recombinant IL 6 did not result in increased survival of mice following lethal irradiation. In fact, such treatment given 20 hrs before LD50/30 doses of radiation resulted in reduced survival of mice. However, IL 6 augmented the radioprotective effect of IL 1. IL 1 in doses above 10 ng/mouse induced within 2 to 6 hrs a dose dependent increase in CSF in circulation, but IL 6 did not induce detectable levels of CSF at 2, 6 and 20 hrs after administration. Administration of IL 6 to mice produced a dose dependent increase in circulating fibrinogen and SAA. Similarly, administration of IL 1 resulted in much greater increases in levels of fibrinogen and SAA. Therefore, IL 1 is a more effective inducer of fibrinogen and SAA in mice than is IL 6. Although definitive conclusions concerning the relative roles for IL 1 and IL 6 in vivo will await availability of anti IL 1 and anti-IL 6 antibodies, our data do not support the suggestion that the above IL 1 effects can be attributed solely to IL 6.
- Research Organization:
- Armed Forces Radiobiology Research Institute, Bethesda, MD (USA)
- OSTI ID:
- 6403039
- Journal Information:
- Lymphokine Res.; (United States), Vol. 7:4
- Country of Publication:
- United States
- Language:
- English
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FIBRINOGEN
BIOSYNTHESIS
LYMPHOKINES
RADIOSENSITIVITY EFFECTS
BONE MARROW
COMPARATIVE EVALUATIONS
DOSE-RESPONSE RELATIONSHIPS
IN VIVO
LETHAL IRRADIATION
MICE
SURVIVAL CURVES
ANIMAL TISSUES
ANIMALS
BLOOD COAGULATION FACTORS
BODY
COAGULANTS
DRUGS
GLOBULINS
GROWTH FACTORS
HEMATOLOGIC AGENTS
HEMATOPOIETIC SYSTEM
HEMOSTATICS
IRRADIATION
MAMMALS
MITOGENS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RODENTS
SYNTHESIS
TISSUES
VERTEBRATES
560152* - Radiation Effects on Animals- Animals