Radiosensitization of hypoxic bacterial cells by nitroimidazoles of low lipophilicity: steady-state and rapid-mix studies
Radiosensitization of hypoxic bacterial cells by five 2-nitroimidazoles, with similar reduction potentials to misonidazole but having lower lipophilicites, has been measured in Escherichia coli AB 1157 and Streptococcus lactis 712. Sensitization efficiency progressively decreased with decreasing lepophilicity in E. coli but not in S. lactis. This difference is discussed in terms of the differing membrane properties of the two bacteria; E. coli resembled a multicompartment model, as would also be expected with mammalian cells. Rapid-mix experiments are described which show that the radiosensitization observed after experiments are described which show that the radiosensitization observed after preirradiation contact times between ca. 3 and 30 msec is dependent on the lipophilicity of the sensitizer, higher lipophilicity resulting in a lower contact time being required for radiosensitization. This result and the observation that a highly lipophilic compound affects only half the full oxygen enhancement level after short contact times suggest that part of the sensitization process occurs in a lipophilic compartment of the cell.
- Research Organization:
- Mount Vernon Hospital, Middlesex, England
- OSTI ID:
- 6395191
- Journal Information:
- Radiat. Res.; (United States), Vol. 85:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ESCHERICHIA COLI
RADIOSENSITIVITY
IMIDAZOLES
RADIOSENSITIVITY EFFECTS
STREPTOCOCCUS
BIOCHEMISTRY
BIOLOGICAL RADIATION EFFECTS
CALCULATION METHODS
CHEMICAL PROPERTIES
COBALT 60
CULTURE MEDIA
AZOLES
BACTERIA
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CHEMISTRY
COBALT ISOTOPES
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
MICROORGANISMS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
RADIATION EFFECTS
RADIOISOTOPES
YEARS LIVING RADIOISOTOPES
560130* - Radiation Effects on Microorganisms
560121 - Radiation Effects on Cells- External Source- (-1987)